应用PCR-DGGE技术分析福州左海湖的细菌群落组成

Composition of Bacterial Community at Lake Zuohai Determined by PCR-Denaturing Gradient Gel Electrophoresis

应用PCR-DGGE技术对4个月份(2011年12月、2012年2月、4月、6月)的福州左海湖细菌群落组成和优势菌群进行分析,研究结果发现相同月份4个采样点的DGGE图谱差异性不大,细菌群落组成具有较高的相似性;而不同月份4个采样点的DGGE条带的数目和位置表现出明显差异,细菌群落组成差异明显。对20条不同位置的DGGE条带进行切胶回收、扩增和测序后,进行系统进化分析,结果显示这20条带归属于4个细菌类群,即变形菌门Proteobacteria、拟杆菌门Bacteroidetes、放线菌门Actinobacteria、蓝细菌门Cyanobacteria。20条序列中有11条鉴定为变形细菌门Proteobacteria、5条鉴定为拟杆菌门Bacteroidetes、2条鉴定为放线菌门Actinobacteria、其余2条属于蓝细菌门Cyanobacteria。研究结果表明,左海湖细菌群落组成包括了Proteobacteria、Bacteroidetes、Actinobacteria、Cyanobacteria,其中以Proteobacteria为优势菌群。

Composition and predominant groups of the bacterial community in Lake Zouhai in the city of Fuzhou were determined and analyzed by using PCR-DGGE. Water samples were collected from 4 sites each time in December 2011, February 2012, April 2012, and June 2012. The results showed that the water samples from the different sites on a same date had a similar bacterial composition, while the numbers and positions of DGGE profiles indicated significant compositional differences on different dales. Twenty I）GGE bands with varied phylotypes were excised, cloned, sequenced, and analyzed to show 11 of them having possible affiliations with Proteobaeteria, 5 with Bacteroidetes, two with Aetinobacteria, and two with Cyanobaeteria. Analysis on the bacterial compositions suggested that Proteobacteria was the predominan! group existed in the lake.