冀北山区野生红柴胡组织培养与快速繁殖研究

Study on Tissue Culture and Rapid Propagation of B.scorzonerifolium Willd. in Mountainous Area of Northern Hebei Province

以冀北山区野生药用植物红柴胡的腋芽为外植体,以MS+6-BA 1.5mg/L+IBA 0.5mg/L为诱导培养基,以MS+6-BA 1mg/L+IBA 0.5mg/L为增殖培养基,以1/2MS+IBA 0.5mg/L为生根培养基,采用渐进法炼苗,注重适时移栽,成活率达到90%以上,且苗齐苗壮。

To establish the tissue culture and rapid propagation system of the wild medicinal plant B.scorzonerifolium Willd.in the mountain area of n orthern Hebei, the axillary buds were used as the explants, and the suitable medium for induction,propagation and rooting was selected. The results showed that the seedlings survival rate was more than 90%, and the seedlings were vigorous through asymptotic method training and transplanting in suitable time when the inducing, proliferation and rooting culture medium was MS＋6-BA 1mg/L＋ IBA 0.5mg/L,MS＋6-BA 1mg/L＋ IBA 0.5mg/L and 1/2MS＋IBA0.5 mg/L respectively.