玉米细胞质雄性不育系的鉴定

Identification of Cytoplasmic Male Sterile Line in Maize

采用田间育性调查、花粉活力检测、特异引物PCR鉴定及细胞学形态观察等方法来研究新育成的雄性不育系DT-合344、ZT-合344的育性特点、胞质类型。结果表明,这两个不育系均为T型细胞质败育类型,该研究结果为进一步深入研究不育系的不育分子机理和生产应用奠定基础。

In order to identify the fertility characteristics and cytoplasmic type of two new cytoplasmic male sterile lines（DT-344、ZT-344）,the methods of fertility investigation in field,the testing of pollen viability,the PCR of specific primer and observation of cytology were used in this study. The results showed that the two new cytoplasmic male sterile lines were all T type cytoplasmic abortion. The results of this study lay the foundation for further researching the molecular mechanism of infertility and production applications.