摘要
实验以植物乳杆菌Lp为实验材料,用荧光定量PCR技术分析16S rRNA、Glyceraldehyde-3-phosphate dehydrogenas、L-lactate dehydrogenase以及rec A protein四个候选内参基因的表达情况。应用两种软件ge Norm和Norm Finder程序对实验结果进行分析。实验结果显示在植物乳杆菌Lp中,稳定值最小的是16S ribosomal RNA,用Norm Finder软件分析,稳定值最小的也是16S ribosomal RNA。揭示16S ribosomal RNA适合做植物乳杆菌正常生长状态下的内参基因。
The fluorescence quantitative PCR was used to detect the gene expression level of four genes(16S r RNA,Glyceraldehyde-3-phosphate dehydrogenas,L-lactate dehydrogenase and rec A protein)in Lactobacillus plantarum Lp. The experimental results were analyzed with two kinds of software ge Norm and Norm Finder program. The result showed that the stable minimum value was 16 s rRNA in the two software. 16 s rRNA was suitable for the reference gene in Lactobacillus plantarum Lp.
出处
《黑龙江八一农垦大学学报》
2016年第5期51-55,67,共6页
journal of heilongjiang bayi agricultural university
基金
黑龙江省自然基金重点项目资助(ZD201207)
黑龙江省博士后专项经费资助(LBH-Q13133)
关键词
实时荧光定量PCR
植物乳杆菌Lp
内参基因
real-time fluorescence quantitative PCR
Lactobacillus plantarum Lp
reference gene