摘要
通过多种测试手段对酸肿牛皮中所提取胶原的性质进行了表征,并与脱灰牛皮中所提取的胶原进行了对比。傅里叶红外、紫外光谱及SDS-PAGE凝胶电泳结果显示,酸肿皮提取的胶原与脱灰皮提取胶原的结构基本一致。微量热差示扫描量热结果显示,酸肿皮提取胶原的热稳定性几乎等同于脱灰皮的。不过,Zeta电位测定表明,酸肿皮提取胶原的等电点降低。扫描电镜观察发现其形貌也发生了变化,通透性变差。成纤维测试结果表明,酸肿牛皮提取胶原的成纤维能力有所下降。
Collagen was extracted from acid- swelling bovine hides（ Col- ABH）,and then the properties were characterized through lots of methods and compared with those of collagen extracted from deliming bovine hides（ Col- DBH）. Results from Fourier transform infrared spectroscopy,ultraviolet- visible spectroscopy and sodium dodecyl sulphate polyacrylamide gel electrophoresis（ SDS- PAGE） indicate that structure of Col- ABH is similar to that of Col- DBH. Ultra- sensitive differential scanning calorimetry results indicate Col- ABH possesses almost the same thermal stability with that of Col- DBH. Nevertheless,Zeta potential curves reveal that the isoelectric point（ p I） of Col- ABH is decreased. The morphology of Col- ABH sponge is also changed and its permeability is poorer. Furthermore,the capacity of fibril formation of Col- ABH is inhibited to some extent.
出处
《中国皮革》
CAS
北大核心
2016年第10期31-35,共5页
China Leather
基金
国家自然科学青年基金项目(21306024)
福建省教育厅科技项目(JB13034)
福建农林大学杰出青年科研人才专项基金项目(XJQ201212)
福建农林大学科研启动基金(6112c0700)
关键词
酸肿牛皮
胶原
提取
热稳定性
成纤维
acid-swelling bovine hides
collagen
extraction
thermal stability
fibril formation
