N-乙酰-L-色氨酸对新生鼠脑缺血缺氧纹状体损伤的保护作用

Protective effect of N-acetyl-L-tryptophan on striatal injury of neonatal rat hypoxic ischemic encephalopathy

目的探讨N-乙酰-L-色氨酸(L-NAT)对新生鼠脑缺血缺氧纹状体损伤的保护作用。方法用出生第7天的SD大鼠制备新生鼠脑缺血缺氧模型,HE染色观察纹状体的形态学变化;免疫荧光染色观察Caspase3、Bcl-2和Bax的表达情况。结果与Sham组相比,HIE组纹状体中神经细胞排列紊乱,且数量明显减少,Caspase-3、Bcl-2、Bax的表达升高;HIE+L-NAT组纹状体细胞的损伤程度减轻,Bax、Caspase-3表达下降,Bcl-2的表达进一步升高。结论 L-NAT可能通过调节Bcl-2家族蛋白的表达减轻纹状体细胞的损伤程度,从而保护新生鼠脑缺血缺氧损伤。

Objective To investigate the protective effects of N-acetyl-L-tryptophan （L-NAT） on neonatal rat striatal injury hypoxic-ischemic encephalopathy（HIE）. Methods The HIE rat model was established with SD neonatal rats on 7 d. The morphological change of striatum was evaluated by HE staining and the expression levels of Caspase3,Bcl-2 and Bax were observed by immunofluorescence staining. Results Compared with the sham group,the expression of Caspase-3,Bcl-2 and Bax in the HIE group was increased, while the neurons in striatum were arranged in disorder, moreover the number was significantly decreased;the injury degree of striatal neurons in the HIE＋ L-NAT group was alleviated,Caspase-3 and Bax expression was decreased and Bel-2 expression was further increased. Conclusion L-NAT protects neonatal rat hypoxic-ischemic cerebral injury possibly by regulating Bcl-2 family protein expression to alleviate the injury degree of striatal neurons.