iASPP基因在卵巢癌中的表达及其临床意义

Expression of iASPP gene in ovarian cancer and its clinical significance

目的检测iASPP在卵巢癌中的表达,探索其在卵巢癌中的致病机制。方法收集2013年5月至2014年12月于常州市妇幼保健院行卵巢肿瘤切除术的患者的癌组织及对应癌旁组织标本共32份,用实时荧光定量聚合酶链反应法检测iASPP mRNA相对表达水平并分析它与卵巢癌临床病理特征的关系。结果卵巢癌组织中iASPP mRNA水平明显高于癌旁组织(P=0.001),并且与卵巢癌病理分期相关(P<0.05),iASPP表达水平与患者年龄、是否绝经、组织学类型、术前有无化疗、有无淋巴结转移、糖类蛋白125及糖类蛋白153表达情况无相关性,其差异无统计学意义(P>0.05)。结论 iASPP mRNA在卵巢癌组织中高表达显示其在肿瘤发生过程中发挥重要作用。

Objective To detect the expressions of iASPP in ovarian cancer and to investigate its pathogenic mechanism in ovarian cancer. Methods Totally 32 pair samples of ovarian cancer tissues and corresponding paracancerous tissues were collected in our hospital from May 2013 to December 2014. The iASPP mRNA relative expression level was detected using real-time PCR （RT- PCR）. Its relation with the clinicopathological characteristics of ovarian cancer was analyzed. Results The iASPP mRNA level in ovarian cancer tissues was significantly higher than that in the paracancerous tissues （P=0. 001） and which was associated with the pathological stage（P〈0.05）. The iASPP expression had no correlation with the age,menopause,histological subtype,preoperative chemotherapy,lymph node metastasis and expression level of CA125 and CA153, the differences were not statistically significant. Conclusion The high expression of iASPP in ovarian cancer tissues reveals its important role in the tumor occurrence process.