HPLC同时测定胜红清咽含片中异嗪皮啶、迷迭香酸、绿原酸的含量

Simultaneous Determination of Isofraxidin,Rosmarinic Acid and Chlorogenic Acid in Shenghong Qingyan Buccal Tablets by HPLC

目的建立高效液相色谱法同时测定胜红清咽含片中异嗪皮啶、迷迭香酸和绿原酸的含量。方法采用DIKMA Diamonsil C18(4.6mm×250mm,5μm)色谱柱,流动相为乙腈(A)-0.2%磷酸溶液(B),梯度洗脱(0~8min,15%A;8~20min,15%A^18%A;20~25min,18%A^22%A;25~40min,22%A;40~50min,22%A^25%A;50~60min,25%A^55%A);流速为1.0m L·min^(-1);柱温30℃;检测波长为334nm,进样量为10μL。结果异嗪皮啶、迷迭香酸和绿原酸分别在11.70~234.00μg·m L-1(r=0.9998)、13.58~271.60μg·m L-1(r=0.9999)、5.656~113.12μg·m L-1(r=0.9999)范围内有良好的线性关系。异嗪皮啶、迷迭香酸、绿原酸的平均回收率分别为100.81%、100.75%、100.17%,RSD(n=9)分别为1.32%、1.43%、1.31%。结论该方法操作准确、简便、重现性好,可有效控制胜红清咽含片的质量。

OBJECTIVE To establish an HPLC method for Simultaneous determination of isofraxidin,rosmarinic! acid and chlorogenic acid in Shenghong Qingyan Buccal tablets. METHODS The DIKMA Diamonsil C18（ 4. 6mm × 250 mm,5μm） was used. The mobile phase was acetonitrile（ A） and 0. 2% phosphoric acid solution（ B）in a gradient elution（ 0 ~ 8min,15% A; 8 ~ 20 min,15% A ~ 18% A; 20 ~ 25 min,18% A ~ 22% A; 25 ~ 40 min,22%A; 40 ~ 50 min,22% A ~ 25% A; 50 ~ 60 min,25% A ~ 55% A）,ata flow rate of 1. 0 m L·min- 1and at 30℃ of column temperature. The detection wavelength was 334 nm. RESULTS The Calibration curves of isofraxidin,rosmarinic acid and chlorogenic acidwere in good linearity over 11. 70 ~ 234. 00μg·m L^（-1）（ r = 0. 9998）,13. 58 ~ 271. 60μg·m L^（-1）（ r = 0. 9999）,5. 66 ~ 113. 12μg · m L^（-1）（ r = 0. 9999）,respectively. Their average recoveries were100. 17% ~ 100. 81%,（ RSD 1. 31%,-1. 43%,n = 9）. CONCLUSION This method was accurate,simple and sensitive and can effectively control the quality of Shenghong Qingyan Buccal tablets.