轴突导向因子-1在胎儿生长受限胎盘组织中的表达及意义

Expression of Netrin-1 in Placenta Tissues of Patients with Fetal Growth Restriction

目的：探讨神经轴突导向因子-1（ netrin-1）在胎儿生长受限患者胎盘组织中的表达变化。方法分析33例胎儿生长受限患者（其中18例为胎儿生长受限合并妊娠期高血压患者）及24例正常妊娠孕妇相关数据，并利用Real time-polymerase chain reaction （ RT-PCR）和Western印迹检测netrin-1在胎盘组织中的表达差异。结果①临床数据显示胎儿生长受限患者与正常孕妇在年龄、孕周、体重指数、血压、胎盘重量和出生体重等方面存在显著差异（ P＜0．05或P＜0．01）；②免疫组化结果显示netrin-1在胎盘中存在表达。③ RT-PCR及蛋白质印迹法显示胎儿生长受限患者胎盘组织中netrin-1的表达明显降低。结论 ne-trin-1表达降低可能是导致宫内胎儿生长受限的机制之一。

Objective To investigate the expression of netrin-1 in placenta tissues of patients with fetal growth restriction （ FGR） . Methods The expression of netrin-1 was detected by immu-nohistochemistry, PCR and Western blotting in placental tissues of 33 FGR patients （ including 18 patients concomitant with pregnancy-induced hypertension） and 24 normal pregnant women. Results① There were significant differences in maternal age, gestational age, body mass index, hyper-tension, placental weight and birth weight between FGR group and normal group （ P〈0. 05, P〈0. 01 ） .② Immunohistochemistry showed that netrin-1 was expressed in placenta tissues. ③ PCR and Western blotting showed that the expression of netrin-1 was significantly reduced in FGR group as compared with control group. Conclusion netrin-1 may be involved in the pathogenesis of FGR.