桶装饮用水中铜绿假单胞菌的检测及耐药性分析

Detection of Pseudomonas aeruginosa in bottled drinking water and analysis of antibiotic resistance

目的了解舟山市桶装饮用水卫生情况。方法依据国标GB/T 8538—2008,对随机抽检的94份桶装饮用水,其中48份桶装饮用纯净水,46份桶装矿泉水进行铜绿假单胞菌的检测与鉴定。采用K-B纸片扩散法,对鉴定出的铜绿假单胞菌进行药敏试验。结果确认有3份水样检出铜绿假单胞菌,检出率为3.2%,其中桶装饮用纯净水检出3份,桶装天然矿泉水检出0份;3份阳性样品中共检测出4株铜绿假单胞菌,污染水平分别为27 cfu/250 ml、38 cfu/250 ml、6 cfu/250 ml、24 cfu/250 ml,其中3株产绿脓菌素,1株产荧光色素不产绿脓菌素。药敏试验结果显示:分离鉴定出的4株铜绿假单胞菌对16种抗生素中的新霉素(NEO)、恩诺沙星(ENR)、氨苄西林(AMP)、复方新诺明(SMZ)、青霉素(PNE)、强力霉素(DOX)6种抗生素均有抗性;对头孢曲松(CEFT)、环丙沙星(CIP)、氧氟沙星(OFL)、庆大霉素(GEN)、诺氟沙星(NOR)、头孢哌酮(CEF)等6种抗生素敏感。结论桶装饮用纯净水中存在铜绿假单胞菌污染,检出的铜绿假单胞菌具有多重耐药性。

Objective To understand the hygiene situation of bottled drinking water in Zhoushan. Methods According to the national standard GB/T8535- 2008,94 bottled drinking water,including 48 bottled drinking water and 46 bottled mineral water,were randomly sampled for the detection and identification of P. aeruginosa. And the detected isolates were conducted for sensitivity test with K- B paper diffusion method. Results Pseudomonas aeruginosa was detected in 3 samples,with the detection rate of 3. 2%,of which 3 cases were detected in bottled drinking water,while 0 case was detected in bottled natural mineral water. 4 strains of Pseudomonas aeruginosa were detected from the 3 cases of positive samples,in which 3 strains produced pyocyanin,the other one strain produced fluorescent pigment not pyocyanin,and the pollution levels were 27 cfu/250 ml,38 cfu/250 ml,6 cfu/250 ml,24 cfu/250 ml respectively. The 4 strains of Pseudomonas aeruginosa were all resistant to NEO,ENR,AMP,SMZ,PNE,DOX and were all sensitive to EFT,CIP,OFL,GEN,NOR,CEF. Conclusion The bottled purified water were contaminated by Pseudomonas aeruginosa,and Pseudomonas aeruginosa was multiple resistant to antibiotics.