人参连作根际土壤中酚酸物质对人参锈腐病菌的化感效应

Allelopathic effects of phenolic compounds of ginseng root rhizosphere on Cylindrocarpon destructans

采用高效液相色谱法对人参连作根际土壤中的酚酸物质进行了分离鉴定,检测发现人参根际土壤中含有没食子酸、水杨酸、3-苯基丙酸、苯甲酸和肉桂酸5种酚酸物质.采用外源添加法研究该5种酚酸物质对人参锈腐病菌的化感效应.结果表明:5种酚酸对人参锈腐病菌的菌丝生长和孢子萌发都表现出高浓度抑制、低浓度促进的作用.没食子酸、水杨酸和苯甲酸在0.5 mmol·L^(-1)处理浓度下,3-苯基丙酸和肉桂酸在0.05 mmol·L^(-1)处理浓度下,均能够显著促进人参锈腐病菌菌丝生长和孢子萌发,并显著加重人参锈腐病病害严重度.

In this study, five phenolic compounds of ginseng rhizosphere soil were identified by HPLC, including gallic acid, salicylic acid, 3-phenylpropionic acid, benzoic acid and cinnamic acid. The results show that five phenolic compounds inhibited mycelium growth and spore germina- tion at high concentration, but promoted mycelium growth and spore germination at low concentration. Gallic acid, salicylic acid, benzoic acid of 0.5 mmol · L-1 and 3-phenylpropionic acid, cinnamic acid of 0.05 mmol · L-1 could significantly promote the spore germination, mycelium growth and disease severity of Cylindrocarpon destructans.