大孔树脂纯化玉竹提取物总黄酮的工艺研究

Research on purification of total flavonoids extracted from polygonatum by macroporous resin

以玉竹提取物为原料,以总黄酮吸附率及解吸率为指标,采用动态吸附—解吸的方法筛选出大孔树脂的类型。通过单因素实验和正交实验确立了D-101树脂吸附玉竹总黄酮的优化工艺条件,洗脱过程考察了主要影响因素洗脱剂浓度及其用量。优化后的吸附工艺条件为:树脂用量55 g、上柱药液浓度40.54μg/m L、上柱液p H 6、吸附流速0.5 BV/h。解吸过程解吸液乙醇浓度和用量分别为60%和100 m L。经D-101大孔树脂分离纯化后,玉竹提取物总黄酮的纯度由0.36%提高到2.05%。该纯化方法低廉、安全、操作简单,有较高的应用价值。

Taking polygonatum extraction as raw material, the adsorption rate and desorption rate of total flavonoids as the indexes, the type of macroporous resin was screened out by dynamic adsorption - de- sorption method. The process condition of absorbing the total flavonoids of polygonatum by D - 101 resin was established by single factor experiments and orthogonal experiment; the main factors, concentration and dosage of eluent, was discussed. The optimal adsorption process condition was ： the amount of macro- porous resin 55 g, the concentration of upstream solution 40.54 μg/mL, pH 6, and adsorption velocity 0.5BV/h. During the process of desorption solution, the concentration of ethanol was 60% and the dos- age of ethanol was 100 mL. After being purified by D - 101 macroporous resin, the purity of total fla- vonoids was increased from 0.36% to 2.05%. This purification method is cheap, safety, simple and has a high application value.