石榴皮多酚提取工艺优化及抑菌活性研究

Optimization of extraction of pomegranate peel polyphenols and research on the bacteriostatic activity

通过单因素实验和响应面分析,研究不同乙醇浓度、料液比、提取时间和提取温度对多酚提取率的影响,探讨乙醇提取石榴皮多酚的最佳工艺。结果表明最佳工艺为:以60%的乙醇溶液作为提取剂,按1∶25 g/m L的料液比,在30℃条件下提取60 min,石榴皮多酚的提取率可高达14.09%。抑菌实验表明,石榴皮多酚对金黄色葡萄球菌和大肠杆菌的最小抑菌浓度为3 mg/m L,此时对应的抑菌圈直径分别为9.36 mm和8.64 mm。

In order to explore the best extraction technology of pomegranate peel polyphenols by ethanol under different conditions, the influences of ethanol concentration, solid - liquid ratio, extraction time and extraction temperature on the extraction yield were researched by the single factor experiments and re- sponse surface analysis. The results showed that： the extraction yield of pomegranate peel polyphenols was 14.09% under the condition of taking 60% ethanol solution as extractant, with solid - liquid ratio 1 ： 25 g/mL, at 30 ℃ extracting for 60 min. The results of bacteriostatic experiment showed that the mini- mum bacteriostasis concentration of pomegranate peel polyphenols against staphylococcus aureus and esche- richia coli, was 3 mg/mL, while the diameters of the inhibition zones were 9.36 mm and 8.64 mm re- spectively.