云南民间常用青叶胆类药材中环烯醚萜苷类成分的含量测定

HPLC Analyzed the Iridoid Glycosides of Qingyedan Commonly Used in Folk Medicine in Yunnan

目的:比较云南民间常用青叶胆类药材中环烯醚萜苷类成分的含量,为青叶胆基原植物药用合理性及资源利用提供依据。方法:采用HPLC法测定青叶胆类药材中獐牙菜苦苷、龙胆苦苷、獐牙菜苷的含量。结果:9种共30批青叶胆类药材成分差异显著。结论:几种药材化学成分因种类和产地不同而差异较大,不宜作为替代药物使用,将獐牙菜苦苷和獐牙菜苷作为质量控制指标,是鉴别和控制青叶胆类药材质量的有效手段。

Objective： To compare the contents of iridoid glycosides in Qingyedan medicinal materials,and to provide the scientific basis for using resources of Qingyedan and rationality of original plant medicinal. Methods： The contens of three iridoid glycosides,including swertiamarin,gentiopicroside and sweroside in Qingyedan medicinal materials were determined by HPLC. Results： The constituents of 30 samples in nine species were significant difference. And the contents of iridoid glycosides in Swertia bimaculata,Swertia tenuis and Swertia pubescens were reported for the first time. Conclusion： The results show that the contents of iridoid glycosides in Qingyedan medicinal materials have a significant difference due to the different species and producing areas. Therefore,these medicinal plants should not be used as alternative medicines for clinical application. Swertiamarin and sweroside can be selected as quality control components,this method is an effective method to identify and control the quality of Qingyedan materials.