沙门氏菌的PFGE分型及耐药分析

Molecular typing of salmonella by using pulsed-field gel electrophoresis and and drug resistance analysis

目的分析2015年乌鲁木齐市腹泻性疾病中沙门氏菌的耐药性和分子分型情况,探讨其多态性及其与分子流行病学的关系,为减少和控制沙门氏菌病的流行提供参考。方法将分离获得的71株沙门氏菌进行血清型鉴定,KB药敏纸片法检测其药物敏感性,采用脉冲场凝胶电泳（pulsed-field gel electrophoresis,PFGE）对分离菌进行电泳分型,所得结果用Bio Numerics 6.6软件进行聚类分析。结果根据电泳指纹图谱,可将71株肠道沙门氏菌分为29个PFGE型别,菌株间的相似值在49.2%-100.0%之间,S-17、S-18型为优势型别,共计43.7%（31/71）,其中23.9%（17/71）的菌株为S-17型,19.7%（14/71）的菌株为S-18型;药敏结果：头孢类和环丙沙星敏感率分别为98.6%（70/71）和95.8%（68/71）。结论脉冲场凝胶电泳对沙门氏菌有较高的分型能力,应充分结合传统实验室检测技术和PFGE技术优势,系统地开展腹泻病中沙门氏菌溯源分析及分子流行病学研究。

Objective The objective of the present study was to document the resistance and the polymorphism of PFGE molecu- lar type of salmonella which isolated from diarrhea cases in 2015, and to explore theirs relationship of molecular epidemiologi- cal, to reduce and control the epidemic of salmonellosis. Methods In this research, the separation of 71 strains of salmonella were identified by serotype identification, the antibiotic susceptibility test was carried out using Kirby -Bauer（ KB）, then the total genomic DNA of the isolates run pulsed -field gel electrophoresis（ PFGE）, the DNA fingerprints of different stains were an- alyzed by Bionumerics6. 6 software to perform clustering. Results The DNA fingerprints of 71 salmonella were belonged to 29 PFGE pattern combinations. Similarity value of these strains was in the range of 49. 2% - 100.0%. 23.9% （ 17/71 ） of them were S - 17, and 19. 7 % （ 14/71 ） were S - 18, there were 43.7 % （ 31/7 ! ） strains classified into S - 17 and S - 18, which were the prevalent types. Drug susceptibility results： cephalosporin and ciprofloxacin were hypersensitive, and the sensitive rates were 98.6% （70/71） and 95.8% （68/71 ） respectively. Conclusion The numerous PFGE patterns of salmonella in Urumqi show Di- versity. There are different evolutionary sources of cloning plants coexist at the same time and region, and no regular distributions in time and region, besides, one serotype salmonella could be found several PFGE pattern combinations. At present, cephalo- sporin and eiprofloxacin are still the main antibiotics to treat salmonella in Urnmqi. The PFGE technology is a good method for mo- lecular typing of salmonella, it is required to systematiely carry out tracing the suspected source of diarrhea cases and study the molecular epidemiological relationship of different salmonella stains, integrating the method of PFGE molecular classification with the traditional technique of laboratory tests.