木香药材的高效液相色谱特征图谱研究

Study on Characteristic Fingerprint of Aucklandia Radix by HPLC

目的:建立木香药材高效液相色谱特征图谱分析方法。方法:采用Diamonsil C_(18)色谱柱(250 mm×4.6 mm,5μm),以甲醇(A)-0.1%甲酸水(B)为流动相梯度洗脱,流速1.0 ml/min,检测波长254 nm,柱温25℃,建立不同产地木香的高效液相色谱特征图谱;采用《中药色谱指纹图谱相似度评价系统》(2004 A版)进行相似度评价。结果:通过对15批次不同产地木香样品的分析,建立了木香药材高效液相色谱特征图谱,确定了6个共有特征峰,并指认了其中2个特征峰的结构,分别为木香烃内酯和去氢木香内酯。结论:本研究建立的木香药材特征图谱的特征性和专属性强,且方法简便、可靠,重复性好,为更好地控制木香药材的内在质量提供了科学依据。

OBJECTIVE： To establish the HPLC characteristic fingerprint for the quality control of Aucklandia Radix.METHODS： Diamonsil C_（18） chromatographic column（ 250 mm × 4. 6 mm,5 μm） was adopted,the mobile phase consists of methanol（ A） and water containing 0. 1% acetic acid（ B） with gradient elution at the flow rate of 1. 0 ml / min. The UV detection wavelength was 254 nm and the column temperature was 25 ℃. The Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine（ version 2004A） was used to calculate the similarity of the HPLC characteristic fingerprint of Aucklandia Radix from different habitats. RESULTS： HPLC characteristic fingerprint was established with six common characteristic peaks based on the analysis of 15 batches of Aucklandia Radix. Two common characteristic peaks were identified as costunolide and dehydrocostus lactone. CONCLUSIONS： The established method shows good characteristics, specificity, and repeatability, which can provide a scientific basis for the identification and quality control of Aucklandia Radix.