二十八烷醇调节大鼠脂质代谢的研究

Regulation of Lipid Metabolism by Octacosanol in Rats

为研究二十八烷醇对高脂饮食诱导的大鼠血脂、肝脏的影响,将SD大鼠分为普通对照组、高脂对照组、二十八烷醇组,每组10只,连续喂养10周。实验中期(4周、8周)、结束(10周)测定血清TC、TG、LDL-C、HDL-C、ALT、AST、TBA含量,分析肝脏脂肪变性情况。结果发现,与高脂对照组相比,二十八烷醇组可显著降低大鼠血清TG含量,在4周、8周和10周分别降低26.81%(p=0.08)、44.77%(p=0.03)和32.22%(p=0.03);降低血清TC含量分别为16.76%(p=0.18)、16.21%(p=0.04)和8.43%(p=0.07);二十八烷醇对大鼠血清HDL-C、体重及日均摄食量均无影响。通过病理切片及肝功分析可知,二十八烷醇可有效预防肝脏脂肪变性,不会损害大鼠的肝功能。结果表明二十八烷醇具有调节血脂、预防肝脏脂肪变性功能。因此,二十八烷醇可作为预防心脑血管疾病的功能性食品成分。

The effect of octacosanol on serum lipids and the liver of rats fed a high-fat diet was investigated. Thirty Sprague-Dawley rats were divided into three groups： normal control group （n=10）, high-fat control group （n=10）, and octacosanol group （n=10）. The content of serum total cholesterol, triglycerides, low-density lipoprotein-cholesterol （LDL-C）, high-density lipoprotein-cholesterol （HDL-C）, aspartate arninotransferase, alanine transarninase and thiobarbituric acid （TBA） were measured at the middle （weeks 4 and 8） and end （week 10） of the experiment, and the condition of hepatic steatosis was analyzed. The results showed that the serum Iriglyceride content of rats in the octacosanol group at weeks 4, 8 and 10 were significantly decreased by 26.81% （p=0.08）, 44.77% （p=0.03） and 32.22% （p=0.03）, respectively, compared to those in the high-fat group. The total cholesterol （TC） content of rats at weeks 4, 8 and 10 in the octacosanol group were significantly decreased by 16.76% （p=0.18）, 16.21% （p=0.043） and 8.43% （p=0.07）, respectively, compared to those the high-fat group. The serum HDL-C level, average body weight, and daily food intake were not affected by octacosanol. Pathological sectioning and liver function analysis indicated that octacosanol could effectively prevent hepatic steatosis without negatively affecting liver function. The results indicate that octacosanol can be used to regulate blood lipids and prevent hepatic steatosis. Therefore, octacosanol can be used as a functional food ingredient in the prevention of cardiovascular and cerebrovascular diseases.