蜂胶醇提物微胶囊的制备及表征

Preparation and Characterization of EEP Calcium Alginate-chitosan Microcapsules

微胶囊具有保护物质免受环境条件影响、掩盖异味和颜色、降低毒性、延长挥发性物质的储存时间,延缓或控制囊心物的释放等功能。本文采用复凝聚技术制备蜂胶醇提物（EEP）海藻酸钠-壳聚糖微胶囊,利用粒度分析仪、激光共聚焦显微镜、红外光谱仪和X射线衍射仪分析、体外释放试验和加速试验等表征微胶囊性状。研究结果表明,制备的EEP微胶囊形状为大小均匀球体,粒径大小为265±25 nm,包埋率和载药量分别达到72.80±3.8%和19.96±2.4%,海藻酸钠和壳聚糖反应形成微胶囊。模拟胃液和肠液条件下体外释放8 h,释放率46.60±0.80%;HPLC分析加速试验EEP微胶囊中7种物质含量变化为14.01%-39.68%,显著低于EEP中37.01%-77.45%。制得的EEP微胶囊粒径小、稳定性良好、释放率符合肠溶体系要求,对EEP中的活性物质具有一定的保护作用。

Microcapsules are capable of protecting substances from harsh environment conditions, covering up odor and color, reducing toxicity, prolonging the storage of volatile substances, and delaying or controlling the release of capsule core materials. Calcium alginate-chitosan microcapsules of the ethanol extract of propolis （EEP） were prepared by complex coacervation and characterized using a particle-size analyzer, confocal laser-scanning microscopy, Fourier transform infrared spectroscopy, X-ray diffraction, a simulated gastrointestinal-tract model, and a temperature-accelerated test. The results showed that EEP microcapsules were spherical, with a mean particle diameter of 265±25 nm, the encapsulation efficiency and loading capacity were 72.80±3.8% and 19.96±2.4%, respectively, and the in vitro release rate over 8 h was 46.60±0.80%. Content changes for seven compounds in EEP microcapsules analyzed by HPLC after a temperature-accelerated test ranged from 14.01% to 39.68%, which was lower than the 37.01% to 77.45% range observed in EEP. The prepared microcapsules exhibited small particle sizes and increased stability, with an appropriate release rate into the enteric system. These results indicated that the active substances encapsulated inside EEP microcapsules were well protected.