MTHFR和MTRR的SNP多态性与汉族孕妇复发性流产的相关性分析

Correlation analysis of SNP polymorphisms of MTHFR and MTRR with recurrent miscarriage of Han Chinese pregnant women

目的探究MTHFR和MTRR的SNP多态性与汉族孕妇复发性流产的相关性。方法选取2012年1月至2015年2月无明确原因复发性流产而至昆山市第四人民医院妇产科就诊的非妊娠妇女190例归为观察组,另外选取同时间段进行体检的健康非妊娠妇女180例为对照组,比较两组妇女MTHFR基因C677T、A1298C和MTRR基因A66G基因位点的单核苷酸多态性（SNP）。结果观察组妇女MTHFR基因C677TT等位基因分布频率为32.11%,对照组为21.94%,观察组显著高于对照组（χ^2=9.647,P=0.002）。观察组妇女M THFR基因A1298C等位基因分布频率为34.47%,对照组为30.83%,两组比较无显著性差异（χ^2=1.113,P=0.291）。观察组妇女M TRR基因A66GG等位基因分布频率为32.37%,对照组为15.28%,观察组显著高于对照组（χ^2=29.558,P=0.000）。结论 M THFR基因C677T位点、M TRR基因A66G位点的多态性与复发性流产密切相关。

Objective To explore the correlation of Single nucleotide polymorphisms（ SNP） polymorphism of MTHFR and MTRR with recurrent miscarriage of Han Chinese pregnant women. Methods Between January 2012 and February 2015 190 non pregnant women with no clear cause of recurrent abortion visiting Kunshan NO. 4 People＇s Hospital were selected in observation group,and 180 healthy non pregnant women taking medical examination at the same period were selected in control group. SNP in C677 T and A1298 C loci of MTHFR gene and A66 G locus of MTRR gene were compared between two groups. Results In the observation group frequency distribution of C677 T allele in MTHFR gene was significantly higher than in the control group（ 32. 11% vs 21. 94%）,and the difference was statistically significant（ χ^2= 9. 647,P = 0. 002）. Frequency distribution of A1298 C allele in MTHFR gene was 34. 47% in the observation group and30. 83% in the control group,and the difference was not significant（ χ^2= 1. 113,P = 0. 291）. In the observation group frequency distribution of A66 G allele in MTRR gene was significantly higher than in the control group with statistical significance（ 32. 37% vs15. 28%,χ^2= 29. 558,P = 0. 000）. Conclusion The polymorphism of C677 T locus of MTHFR gene and A66 G locus of MTRR gene is closely related to recurrent abortion.