淄博地区新生儿网织红细胞参考区间建立的调查研究

Investigation and study of newborn reticulocyte reference range establishment in Zibo area

目的：探讨淄博地区新生儿网织红细胞（reticulocyte，RET）百分比的参考区间。方法：纳入淄博市中心医院2013年1月至2015年6月期间出生的2749例新生儿，其中男婴1408例，女婴1341例，平均日龄4．58天。应用SysmexXN-2000血液全自动分析仪检测RET百分比。RET计数的统计结果用x±s表示，用双侧x±1．96s表示RET的参考区间。按照新生儿日龄分为9组，应用独立样本t检验比较男女新生儿间RET水平差异，应用单因素方差分析比较不同日龄新生儿组间RET水平差异。结果：男、女新生儿RET百分比分别为（2．917±0．273）％、（3．321±0．287）％，差异有统计学意义（t=-2．559，P〈0．05）。不同日龄新生儿RET检测结果差异有统计学意义（F=130．179，P〈0．01）；新生儿RET参考值随着日龄增加而增加，在3日龄时RET参考值最高，随后RET参考值下降。同日龄男、女RET参考值比较，除第5日组男新生儿RET参考值为（2．617±0．073）％与女新生儿RET参考值为（3．528±0．151）％相比较差异有统计学意义t=-2．017，P〈0．05），其他日龄组男、女新生儿RET参考值相比较无统计学意义。结论：通过本次研究建立了淄博地区新生儿RET参考区间，为临床疾病诊断及治疗提供了实验室依据。

Objective： To explore the RET reference range of newborns in Zibo area. Methods： 2749 （1408 males and 1341 females） healthy newborns born in Central Hospital of Zibo from January 2013 to June 2015 were selected, and the samples were divided into nine groups according to different day ages. The RET percentage was counted with XN-2000 automatic hematology analyzer. SPSS 18.0 was used to analyse the collected data. Results： The normal RET reference values of male and female newborns were 2.917%±0.273%, 3.321%±0.287% respectively. PET of male newborns was lower than that of female newborns, the difference was statically significant （t=-2.559,P〈0.05） . The RET difference of different day ages of male and female newborns was statistically significant （F=130.179, P〈0.01） . The comparison of the same day newborns proves that there is PET difference between the male, female newborns of fifth day groups, respectively （2.617±0.073） % , （3.528±0.151） %. There is statistically significant （t=- 2.017,P〈0.05） . PET reference of newborn increases with age, and the RET reference is highest in the third day. ConclusioN： The PET reference of neonatal in Zibo area was established by the study, and to provide laboratory basis for clinical diagnosis and treatment.