四种小血管吻合方法动物实验比较研究

Comparative study of four kinds of small vessel anastomosis methods in animal experiment

目的对比分析四种小血管吻合方法的应用效果。方法 80只Wister大鼠随机分成4组,分别采用端端间断吻合法、等距对称分叶法、小血管褥式套接法以及三针缝合套入法等吻合方法,各20只。统计吻合时间及血管通畅率,并于术后不同时间采用光镜观察吻合口部的组织形态学改变。结果 4组术后血管吻合口即刻通畅率均为100%。远期通畅率:间断缝合组为94.05%,等距对称分叶组为97.5%,褥式套接组为95%,三针缝合套入组为100%。组间差异无统计学意义(P>0.05)。三针缝合套入组吻合时间较其他三种吻合方法短,差异有统计学意义(P<0.05)。术后血管组织形态学改变:三针缝合套入组血管内膜损伤小、管壁各层次结构清晰、炎症反应轻。结论三针缝合套入法吻合小血管手术耗时短,术后血管壁损伤小、炎症反应轻、恢复快。

Objective To compare the effects of four kinds of small vessel anastomosis methods in animal experiment. Methods 80 Wister rats were randomly divided into four groups, 20 cases in each group. The end of the interrupted suture method, equidistant symmetrical lobulation, small vascular mattress suture and three - pin suture method and other anastomosis were used respectively. The time of anastomosis and the patency rate of blood vessel were observed. The histomorphological changes of the anastomotic region were observed by light microscope at different time. Results The immediate patency rates of the four groups after operation all were 100%. The long -term patency rate was 94.05% in the interrupted suture group, 97.5% in the equidistantly lobulated group, 95% in the mattress suture group and 100% in the three -pin suture group. There was no sta- tistical difference （ P 〉 0.05 ）. The average anastomosis time ： anastomosis time of three - pin suture group was shorter than the other three methods of anastomosis, and there was a statistical difference（P 〈 0.05 ）. The morphological changes of the vascular tissue after the operation ： in three - pin suture group, vascular intima injury was light, the wall of each layer was clear, and the inflammatory reaction was mild. Conclusion Three - pin suture method is a good choice for small vessel anastomosis, with short operation time, light vascular intima injury, mild inflammation and fast recovery.