长链非编码RNAuc.339在非小细胞肺癌中的表达及临床意义

Expression and clinical significance of long non-coding RNA uc.339 in non-small cell lung cancer

目的:分析uc.339在非小细胞肺癌中的表达及其与临床病理特征的关系。方法:非小细胞肺癌新鲜标本及其癌旁肺组织各30例,提取总RNA,采用实时定量PCR方法检测uc.339在非小细胞肺癌组织及其癌旁肺组织中的表达,分析差异性及其与临床病理特征的关系。癌性胸腔积液及非癌性胸腔积液各20例,离心沉淀,提取总RNA,采用实时定量PCR方法检测uc.339在癌性胸腔积液及非癌性胸腔积液中的表达;免疫组织化学检测P53在非小细胞肺癌中的表达。结果:uc.339在非小细胞肺癌组织中的表达低于癌旁肺组织(P<0.05),与临床分期有相关性(P<0.05),与p53的表达呈负相关(P<0.05),而与肿瘤病理类型、年龄、性别无明显相关性(P>0.05);uc.339在癌性胸腔积液中的表达低于非癌性胸腔积液(P<0.05)。结论:uc.339可能作为一种抑癌因子作用于非小细胞肺癌的发生、发展中,其作用机制可能与p53信号通路有关,在作为肿瘤标志物、判断预后及靶向治疗等方面具有应用价值。

Objective：To investigate the expression of uc.339 and its association with clinicopathologic features in non-small cell lung cancer （NSCLC）. Methods：The total RNA in 30 fresh specimens of NSCLC and the adjacent normal lung tissues were extracted respectively. hTe expressions of uc.339 in the tissues were detected by real-time quantitative PCR. hTe difference and its relationship with clinicopathological features were analyzed. hTe total RNA in the centrifugation precipitation of 20 specimens of NSCLC pleural effusions and 20 specimens of non-cancer pleural effusions were extracted respectively. The expressions of uc.339 were detected by real-time quantitative PCR. Expressions of P53 in NSCLC tissues were detected by immunochemistry. Results：hTe expression level of uc.339 in NSCLC tumor tissues was much lower than that in the normal tissues （P〈0.05）, which was signiifcantly correlated with clinical stage （P〈0.05）, while was not associated with the histological type, age and gender of the patients （P〉0.05）. A strongly negative correlation was observed between uc.339 and p53 expression （P〈0.05）. hTe expression level of uc.339 in malignant pleural effusions was signiifcantly lower than that in the benign pleural effusions （P〈0.05）. Conclusion：uc.339 might act as an anti-oncogene in NSCLC, its mechanism may be related to p53 signaling pathways. uc.339 plays a role in tumor makers, prognosis and target therapy.