睾丸孤核受体4(TR4)促进巨噬细胞向前列腺癌浸润

Testicular orphan receptor 4(TR4) promotes human macrophage infiltration in prostate cancer

目的:研究前列腺癌TR4水平对巨噬细胞浸润的影响。方法:用transwell体系进行巨噬细胞THP-1迁移实验,在人体组织中研究睾丸孤核受体4(Testicular orphan receptor 4,TR4)水平和巨噬细胞标志物CD36的关系。结果:过表达TR4促进了巨噬细胞向前列腺的迁移;敲低TR4抑制了巨噬细胞向前列腺癌的迁移;人体前列腺癌组织标本中TR4较高的患者,CD36表达较高。结论:前列腺癌的TR4可能促进了巨噬细胞浸润。

Objective：To investigate the role of testicular orphan receptor 4 （TR4） in the recruitment of human macrophage THP-1 cells in prostate cancer. Methods：Transwell system was used in the study of cellular migration to measure the THP-1 recruitment ability of prostate cancer （PCa） cells with varying levels of TR4;human PCa tissue was collected to study the relation of TR4 levels and CD36 levels by IHC staining. Results：Over-expression of TR4 increased THP-1 migration in PCa cells, knocking-down TR4 could decrease the THP-1 migration in PCa cells;IHC staining demonstrated that TR4 levels positively correlate with CD36 levels. Conclusion：TR4 may promote THP-1 cells inifltration in prostate cancer.