多发性内分泌腺瘤病1型相关的甲状旁腺肿瘤p27^KiP1及β-连环蛋白的表达

The expression of p27^Kip1 and β-catenin in multiple endocrine neoplasia type 1-related parathyroid tumors

目的 分析p27^Kip1及β-连环蛋白（β-catenin）在多发性内分泌腺瘤病1型（MEN1）相关的甲状旁腺肿瘤（MHPT）组织中的表达,以探索其在MHPT发病机制中的作用.方法 收集2002年至2013年北京协和医院收治的31例MHPT患者的MHPT组织31份以及5份正常甲状旁腺（NP）组织的石蜡标本,应用免疫组织化学染色法检测p27^Kip1及β-catenin的表达情况.结果 MHPT组织中,p27^Kip1在细胞核表达为缺失、弱阳性或中等阳性（分别为12.9％、32.3％、54.8％）;细胞膜β-catenin表达为强阳性或中等阳性,细胞质β-catenin表达为中等阳性或弱阳性,未见有β-catenin表达于细胞核.MHPT组织p27 Kip1表达水平显著低于NP组织（P=0.001）,但细胞膜及细胞质β-catenin表达在MHPT组织与NP组织差异无统计学意义（P值分别为0.087,0.357）.结论 MHPT组织p27^Kip1蛋白表达显著减少,未发现β-catenin异位聚集于细胞核现象.

Objective To explore tissue expression of cyclin-dependent kinase inhibitor p27^Kip1 and β-catenin in multiple endocrine neoplasia type1 （MEN1）-related parathyroid tumors （MHPT）.Methods Immunohistochemistry was performed to analyze the expression of p27^Kip1 and β-catenin in parathyroid glands from 31 subjects with MHPT collected at Peking Union Medical College Hospital from 2002 to 2013.Five normal parathyroid glands were used as control.Results In MHPT subjects,nuclear expression of p27^Kip1 was absent in 4 （12.9%）,weak in 10 （32.3%） and moderated staining in 17 parathyroid specimens （54.8%）,respectively.While,in normal subjects,the nuclear expression of p27^Kip1 was observed in all subjects and was stronger than that from MHPT subjects （P =0.001）.As to the expression of β-catenin,normal parathyroid showed a distinct to moderate membrane staining,a moderate to weak cytoplasmic staining and negative nuclear staining.Similarly,MHPT exhibited a marked to moderate membrane （P =0.087）,a moderated to weak cytoplasmic （P =0.357）,and negative nuclear β-catenin staining.Conclusions The expression of p27gip1 is reduced or absent in MHPT tissue,and no nuclear expression of β-catenin is observed in the tumors,which suggesting p27^Kip1,but not β-catenin nuclear accumulation,play a role in the development of the tumors.