基于颜色主波长与补色波长的比色法定量检测

Colorimetric Analysis for Quantitative Detection Based on the Dominant Wavelength and the Complementary Wavelength of Colors

利用sRGB（Standard RGB）颜色空间与国际照明委员会（CIE）色度系统的转化关系以及CIE 1931色品图的性质,编写MATLAB程序实现了颜色的RGB信息到颜色主波长或补色波长的转化,并将此程序用于分析面光源下采集到的显色产物的图片。利用颜色的主波长实现了对pH值的定量检测,利用颜色的补色波长完成了对亚硝酸根离子的定量检测。当pH值分别在4.0~7.0和7.5~10.0范围内,pH值均与其显色产物颜色的主波长呈线性变化;亚硝酸根离子浓度在10~40 mg/L范围内与其显色产物颜色的补色波长有良好的线性关系,通过结合图片的G值分析,扩大了传统吸收光谱法的检测范围。

According to the conversion of sRGB （ Standard RGB） color space and CIE （ Commission Internationale de L＇Eclairage） colorimetric system and the character of the CIE 1931 chromaticity dia- gram, the MATLAB program was composed to achieve the conversion from the RGB information to the dominant wavelength or the complementary wavelength of one color, and then was utilized to analyze the images of the chromogenic products under the area light source. The quantitative detection of the pH value was realized with the color＇s dominant wavelength and the detection of the nitrite with the color＇s complementary wavelength. There are linear relationships between pH value and the color＇s dominant wavelength of its chromogenic products in the pH value ranges of 4. 0 - 7.0 and 7.5 - 10. 0, respectively. And there is a favorable linear relationship between concentration（ 10 -40 mg/ L） of nitrite and color＇s complementary wavelength of its chromogenic products. Combined with the Green value of the color, the range of detection by the traditional absorption spectrometry was enlarged.