猪脂肪沉积候选基因AOC3、PPARG1及SOD3 DNA甲基化差异研究

Study on Methylation Difference of Fat Deposition Candidate Genes AOC3,PPARG1 and SOD3 in Pig

试验旨在探究AOC3、PPARG1及SOD3基因的DNA甲基化程度差异对肌内脂肪(intramuscular fat,IMF)表型的影响,以明确甲基化在脂肪沉积过程中的作用。以大白猪×民猪设计资源群体F2代IMF含量表型值极高值与极低值个体为研究对象,选取背最长肌组织,采用亚硫酸盐测序法(bisulfite sequencing PCR,BSP)对AOC3、PPARG1及SOD3基因的CpG岛(CpG island,CGI)进行了DNA甲基化程度检测,用以分析甲基化程度与IMF性状的关联性。结果发现,3个候选基因的检测区段DNA甲基化程度在IMF极高组、极低组间均未检测到明显差异,表明该群体内的IMF含量表型差异可能并不是由这3个候选基因DNA甲基化程度的变化引起的,这些候选基因影响猪肌内脂肪表型的分子机制尚需进一步的研究。

This reasearch intended to find out the link between intramuscular fat（IMF）and DNA methylation level of AOC3,PPARG1 and SOD3 genes,and to make clear how DNA methylation influcence the fat deposition process.We used 10F2 pigs with the extreme IMF of the Yorkshire pig×Min pig resource population.Longissimus muscle tissues were collected to find out the connection between the methylation level and IMF by using BSP.The results showed that there were no significant methylation differences in all the three candidate genes between the IMF extreme groups,which suggested that the phenotypic differences in this population might not be controlled by the degree of the methylation level,further study was still needed.