一个水稻小热休克蛋白基因的克隆和鉴定

Cloning and Characterization of a Small Heat Shock Protein(SHSP)Gene in Rice Plant

小分子热休克蛋白(SHSP)广泛存在于各类生物体中且在生物发育和逆境响应过程中发挥重要作用。我们克隆了一个水稻小热休克蛋白基因(OsSHSP17.6),序列分析表明该基因编码区为477bp,可编码一个含158个氨基酸的多肽,分子量约17.6kD。序列分析显示该类SHSP在不同的植物中是保守的,在分类上隶属于CI类。实时定量RT-PCR分析显示热激处理可显著增强该基因的转录;Western blotting分析显示该蛋白大小与预期一致,且其含量在热激处理时大幅度增加。这些结果一致表明OsSHSP17.6可能参与了水稻热应激反应,这为进一步鉴定OsSHSP17.6的功能提供了基础。

Small heat shock proteins（SHSPs）exist widely in all kinds of creatures and play an important role in both development and stress responses.A gene encoding a SHSP（OsSHSP17.6）was cloned from Oryza sativa and the sequence assembly showed the encoding region of OsSHSP17.6was 477 bp in length,encoding apolypeptide of 158 aa with a molecular weight of 17.6kD.Sequence analysis showed that such kind of SHSPs was conserved in different plants and belonged to the cytosolic class I（CI）.The transcriptional level of OsSHSP17.6 was significantly up-regulated by heat shock with qRT-PCR.The protein appeared to be accumulated up to higher level under the condition of heat shock in western blotting assays.Taken together,these findings consistently indicated that OsSHPS17.6could be involved into the response to heat shock,which could contribute to understanding the function of OsSHSP17.6.