温润辛金培本中药复方对变应性鼻炎豚鼠的影响

Effects of Chinese medicine compound of warming and moistening lung with tonifying spleen on guinea pigs with allergic rhinitis

目的研究温润辛金培本中药复方对变应性鼻炎(AR)豚鼠行为学、血清免疫球蛋白E(Ig E)、白细胞介素-6(IL-6)以及鼻黏膜组织病理学的影响。方法 SPF级豚鼠,雌雄各半,随机分为正常组,模型组,鼻炎康组(0.41 g/kg),氯雷他定组(0.91 mg/kg),温润辛金培本中药复方高剂量组(3.04 g/kg)、中剂量组(1.52 g/kg)、低剂量组(0.76 g/kg)。除正常组外,其余豚鼠以10%的2,4-甲苯二异氰酸酯(TDI)橄榄油溶液双侧鼻孔滴鼻(每侧5μL),每日1次,连续7 d。模型成功后,给药14 d,TDI改为隔日滴鼻1次。观察各组行为学并进行评分,酶联免疫吸附法(ELISA)检测血清Ig E、IL-6的含量,光学显微镜下观察鼻黏膜组织病理学变化,并进行嗜酸性粒细胞(EOS)计数。结果温润辛金培本中药复方中、高剂量组明显降低豚鼠行为学评分,与模型组比较P<0.01或P<0.05。温润辛金培本中药复方各剂量组均明显降低豚鼠血清Ig E、IL-6水平,与模型组比较P<0.01或P<0.05。温润辛金培本中药复方中、高剂量组明显减轻鼻黏膜EOS浸润,与模型组比较P<0.01或P<0.05。温润辛金培本中药复方高剂量组黏膜上皮完好,未见炎性细胞浸润,与模型组比较P<0.05。结论温润辛金培本中药复方可改善AR豚鼠行为学,降低豚鼠血清Ig E、IL-6水平,减轻鼻黏膜病理变化。

Objective To study the effects of Chinese medicine compound of warming and moistening lung with tonifying spleen（ hereinafter＂TCM＂） on behavior,serum IgE and IL-6,as well as nasal mucosa pathological changes of guinea pigs with allergic rhinitis. Methods SPF grade guinea pigs,half male and half female,were randomly divided into normal group,model group,Biyankang group（ 0. 41 g /kg）,loratadine group（ 0. 91 mg / kg）,TCM high-dose group（ 3. 04 g / kg）,mid-dose group（ 1. 52 g /kg） and low-dose group（ 0. 76 g / kg）. Except the normal group,other groups were followed by bilateralnostrils intranasal inhalation once daily with 10% TDI olive oil solution（ 5 μL each nostril）,7 days in a row. After the model was established successfully,the guinea pigs were i. g. corresponding medication,with dripping TDI continuously for 14 days every other day. Nasal symptoms of all groups were observed and recorded. The serum contents of IgE and IL-6 were determined by ELISA,nasal mucosa pathological morphological changes were observed under light microscope,and number of eosinophils（ EOS） were counted. Results Compared with the model group,scores of behavioral symptom were significantly lower in both TCM high- and mid- dose group（ P〈0. 01 or P〈0. 05）,and serum content of IgE and IL-6significantly lower in all the TCM groups（ P〈0. 01 or P〈0. 05）. High-dose and mid-dose of TCM relieved EOS infiltration of nasal mucosa,compared with model group（ P〈0. 01 or P〈0. 05）,furthermore,in TCM high dose group,no exposure-related nasal lesions or no flammatory cell infiltration was found（ P〈0. 05）. Conclusion TCM could improve the behavior of guinea pigs with allergic rhinitis and reduce serum IgE and IL-6 levels,relieve the nasal mucosa pathological changes.