蒙药复方述达格-4有效部位抗实验性胃溃疡的作用及其机制

Effect and mechanism of effective components of Mongolian medicine compound Shudage-4 against experimental stomach ulcer

目的观察蒙药复方述达格-4有效部位对冰乙酸导致胃溃疡的影响,探讨其对胃溃疡的治疗作用及机制。方法健康SD大鼠随机分成蒙药复方述达格-4有效部位组(高、中、低剂量组)、阳性对照组(安胃疡组和西咪替丁组)、模型组、正常组,除正常组外,其他组采用冰乙酸致胃溃疡模型,模型成立后各给药组分别灌胃给药14 d,末次给药后24 h处死大鼠,取胃组织检查溃疡指数、计算溃疡抑制率;用ELISA试剂盒测定胃黏膜中一氧化氮(NO)和脂质过氧化产物丙二醛(MDA)含量;用苏木素-伊红(H-E)染色处理胃组织切片,光镜观察组织病理学改变。结果蒙药复方述达格-4高、中、低剂量组均能使胃黏膜中NO含量升高、MDA水平降低,与模型组比较存在显著性差异(P<0.01),同时蒙药复方述达格-4高、中、低剂量组较模型组炎性特征均减轻,中剂量组与高剂量组效果较好,炎细胞数量明显减少并能明显改善病理状况。结论蒙药复方述达格-4对冰乙酸型胃溃疡具有治疗作用,其抗胃溃疡作用机制与抗氧化、舒张血管作用有关。

Objective To explore the protective effect and mechanism of Mongolian medicine compound Shudage-4 on gastric ulcer induced by glacial acetic acid. Methods Healthy SD rats were randomly divided into Shudage-4 high,middle and low dose（ high-,mid-,and low-dose） group,positive drug groups（ Anweiyang group and cimetidine group）,model group and normal group. Except for the normal group,the rats in the other six groups were modeled by glacial acetic acid. After the model was established successfully,rats of each group were admistered i. g corresponding medicine for consecutive 14 days. Then the gastric tissue was resected 24 h after the last administration. Ulcer index and ulcer inhabitation rate were measured,the content of NO and MDA in gastric mucosa were detected using ELIAS kits,and pathological changes of gastric tissue was stained by HE were observed under light microscope. Results Compared with the model group,the content of NO in gastric mucosa increased significantly and MDA decreased significantly in all three Shudage-4 groups（ P〈0. 01）. The inflammation decreased in all three Shudage-4 groups,in which mid-group and high-group showed more obvious effect with much less the number of inflammatory cells and improvement of the pathological condition.Conclusion Mongolian medicine compound Shudage-4 has the therapeutic effect on gastric mucosal injury induced by glacial acetic acid in rats. The mechanism may be related to its effect of antioxidation and vasodilation.