有氧运动预防及治疗ApoE^(-/-)小鼠动脉粥样硬化过程中脂联素的抗炎症作用

The anti-inflammatory effects of adiponectin on the anti-atherosclerosis in ApoE^(-/-) mice after aerobic exercise

目的:观察有氧运动对Apo E^(-/-)小鼠动脉粥样硬化(AS)形成的预防作用和对已形成的AS的治疗效果,探讨此过程中脂联素对抗主动脉炎症的作用机制。方法:40只8周龄雄性Apo E^(-/-)小鼠随机分为14周模型组(C14,10只)、14周运动组(E14,10只)、26周模型组(C26,10只)、14周模型组+12周运动组(E12,10只,造模16周后运动训练12周)。4组小鼠均饲以高脂膳食饲料,E14、E12两组小鼠进行跑台运动(13m/min×60min,跑台坡度为0°,每周5次)。运动结束取材比较4组小鼠主动脉管壁的病理变化,检测比较其脂肪组织脂联素m RNA表达水平、血清脂联素水平及主动脉NF-κB、VCAM-1、SR-A、CD36m RNA表达及蛋白表达水平。结果:与C14组小鼠相比,E14组小鼠主动脉管壁纤维弹力板和内膜较完好,C26组小鼠较C14组主动脉病变进一步发展,E12组小鼠较C26组小鼠主动脉病变有所延缓;E14组小鼠脂肪组织脂联素m RNA表达和血清水平显著高于C14组(P<0.01),E12组小鼠脂肪组织脂联素m RNA表达和血清水平显著高于C26组(P<0.01);E14组小鼠主动脉NF-κB、VCAM-1、SR-A m RNA表达和蛋白表达均显著低于C14组(P<0.01),E12组小鼠主动脉NF-κB、VCAM-1、SR-A m RNA表达和蛋白表达水平显著低于C26组(P<0.05),主动脉CD36 m RNA表达和蛋白表达未发生显著变化。结论:有氧运动提高了Apo E^(-/-)小鼠脂肪组织脂联素m RNA表达及血清脂联素水平,降低了小鼠主动脉NF-κB、VCAM-1、SR-A m RNA及蛋白表达,而且无论是对预防实验组小鼠还是治疗实验组小鼠都有明显的效果,这可能是脂联素在有氧运动预防及治疗Apo E^(-/-)小鼠AS过程中的抗炎症作用机制之一。

Objective：To observe the therapy and prevention effect of exercise on the ApoE~ mice athero sclerosis（AS） formation and speculate the mechanism of anti-inflammatory effects of adiponectin on aortitis Method： Forty 8-week-old ApoE-/- mice were randomly assigned to four groups： 14-week model group（C14,n= 10）, 14-week exercise group（El4, n=10）, 26-week model group （C26,n=10）, 12-week exercise group （E12,n=10, 16 weeks molding followed by 12 weeks exercise）.The molding mice were fed with high fat diet. Mice in the aerobic exercise-trained groups ran on a treadmill （13m/min×60min, 5 days/week）. At the end of experiment, atherosclerosis. Adiponectin mRNA in adipose tissue, serum adiponectin. NF-rB,VCAM-1,SR-A, CD36 mRNA and protein in aorta were detected and compared between the 4 groups. Result： Elastic layer and tunica intima of vascular wall of El4 group was significantly better than Cl4 group, C26 group was more serious than C14 group, El2 group was better than C26 group. Adiponectin-mRNA and serum Adiponectin in the adipose tissue of exercise-trained group were significantly higher than that of the model group （0.61±0.10mg/L vs 0.42±0.12mg/L and 0.49±0.11mg/L vs 0.30±0.08mg/L,P〈0.01）. NF-kB,VCAM-1, SR-A mRNA and protein in aorta of exercise-trained group were significantly lower than that of the model group too （0.94±0.23 vs 1.22±0.30,0.84±0.13 vs 1.23±0.22,0.59±0.08 vs 1.13±0.19,P〈0.01 and 1.28±0.24 vs 1.43±0.19,1.33±0.18 vs 1.52±0.28,1.22±0.15 vs 1.38±0.12,P〈0.05）. Expression of CD36 mRNA and protein in aorta had no significantly difference. Conclusion： Aerobic exercise significantly increase expression of adiponectin-mRNA in adipose tissue, serum ad- iponectin in the ApoE-/- mice, lowered NF-kB,VCAM-1,SR-A mRNA and protein in aorta. Those effects were significant for both prevention group and therapeutic group. Increasement of adiponectin is likely to be one of the factors to anti-atherogenesis in Aerobic exercise.