组织工程技术复合修复兔口腔黏膜的实验研究

Repairmen of the rabbit's oral mucosa by tissue engineering technology

目的:探讨骨髓间充质干细胞(BMMSCs)与左旋聚乳酸/丝素蛋白(PLLA/SF)支架复合物修复兔口腔黏膜的可行性,同时评估PLLA与SF以何种比例混合构建组织工程口腔黏膜支架最理想。方法:分离、培养并鉴定BMMSCs,将其置于PLLA与SF的质量配比为70∶30(P70S30)PLLA/SF支架上复合培养1周后观察细胞表型有无改变;观察BMMSCs与P70S30的PLLA/SF支架复合物的口腔黏膜移植1周后的结构变化;比较细胞与支架复合物组、单纯支架组、创面自然修复组的创面愈合率。结果:1PLLA/SF支架对BMMSCs的细胞表型无明显影响;2BMMSCs与P70S30的PLLA/SF支架复合物口腔黏膜移植1周后与创面的融合较好;3以PLLA与SF的质量配比为70∶30的复合物组愈合率最高,平均愈合率为94.8%,与创面自然修复组及单纯支架组比较差异有统计学意义(P<0.01)。结论:1BMMSCs与PLLA/SF支架复合物具有构建理想的组织工程口腔黏膜的潜力;2PLLA与SF的质量配比为70∶30时最利于口腔黏膜创面的愈合,构建理想的组织工程口腔黏膜的潜力最大。

Objective：This experiment proposed to complicate BMMSCs and PLLA/SF scaffolds,to study its repairing ability for rabbit oral mucosa wound,and try to evaluate the most potential mixed proportion of PLLA and SF for tissue engineering.Method：Separating,cultivating and identifying BMMSCs.Observing cell phenotype of BMMSCs after cultivating BMMSCs in P70S30PLLA/SF scaffold for one week.Observing the structure change of BMMSCs and P70S30PLLA/SF scaffold complexes after oral mucosa transplantation experiment for a week;Comparing the wound healing rate of composite of BMMSCs and PLLA/SF scafold,PLLA/SF scaffold and nature repair.Result：1There wasn＇t a significant effect of PLLA/SF scaffold on cell phenotype of BMMSCs.2The wounds fused well with the oral mucosa transplanted composite of BMMSCs and P70S30PLLA/SF scaffold after a week.3The healing rate of composite of BMMSCs and P70S30 scaffold was the highest.The average healing rate of composite of BMMSCs and P70S30 scaffold was 94.8%,whose difference was statistically significant compared with natural healing and pure scaffold（P〈0.01）.Conclusion：1The complex composed of BMMSCs and PLLA/SF scaffolds has the potential as an ideal scaffold for tissue engineering oral mucosa.2The complex composed of BMMSCs and P70S30PLLA/SF scaffolds is the most beneficial to wound healing of oral mucosa,and has the greatest potential for constructing tissue engineering oral mucosa.