间接免疫荧光抗体技术检测猪肺炎支原体

Indirect Immunofluorescence Antibody Technique to Detect the Pig Pneumonia Mycoplasma

研究介绍了间接荧光抗体技术检测猪肺炎支原体的方法。具体操作为:先进行Hep-2细胞和猪肺炎支原体菌株的培养,然后进行间接荧光抗体检测试验,确定抗体工作浓度之后,取病料刮取菌液进行菌株检测。结果表明,检测结果与临床诊断结果一致,这种方法可以用于难确诊病例的诊断,同时根据黏附计数,可以推断感染菌株的毒力,为临床诊断提供有力的依据。

This article introduces the Hep-2 cells adhesion pig pneumonia myeoplasma, and uses indirect fluorescent antibody technique to detect the pig pneumonia mycoplasma. Specific operation is to Hep-2 cells and pigs, the cultivation of pneumonia myeoplasma strains, and indirect immunofluoreseence antibody testing experiment, determine the antibody working concentration, scrape a gas-bacilli take epidemic materials according to the determination of the concentration in front of the tested strains. The result shows that test results are consistent with clinical diagnosis, so this method can be used in the diagnosis of difficult cases confirmed, at the same time according to the attached count can infer that infected with strains of virulence.