皮肤再生医疗技术对大鼠慢性难愈合创面组织匀浆中TNF-α和IL-6水平的影响

Effect of MEBT/MEBO on expression of TNF-α and IL-6 in tissue homogenate of chronic non-healing skin wound in rats

目的观察皮肤再生医疗技术(MEBT/MEBO)对大鼠慢性难愈合皮肤创面组织匀浆中肿瘤坏死因子α(TNF-α)、白细胞介素6(IL-6)水平的影响。方法将54只慢性难愈合创面模型SD大鼠随机分为观察组、贝复济组、模型组各18只,造模成功后以湿润暴露疗法(MEBT)创面分别贴两层湿润烧伤膏(MEBO)纱条、贝复济浸透纱布、生理盐水纱布;空白组建立全层皮肤缺损模型,不加任何干预措施。造模后第3、7、14天各组随机处死5只大鼠,取相同部位皮肤溃疡创面肉芽组织,采用HE染色观察创面组织病理,ELISA法检测创面组织匀浆中的TNF-α、IL-6水平。结果造模后第14天,观察组、贝复济组大鼠创面组织中大量微血管生成,炎症细胞消失,创面恢复良好。各组大鼠创面组织匀浆中TNF-α、IL-6水平随时间延长均呈下降趋势,但以观察组下降最明显,模型组下降最缓慢;观察组、贝复济组创面组织匀浆中TNF-α、IL-6水平始终高于空白组(P均<0.01);且造模后第7天,观察组创面组织匀浆中TNF-α、IL-6水平低于贝复济组(P均<0.01);造模后第14天,观察组与贝复济组创面组织匀浆中TNF-α、IL-6水平比较无统计学意义(P均﹥0.05)。结论 MEBT/MEBO可以合理控制炎症因子TNF-α、IL-6的释放,改变病理炎症反应状态,从而促进创面愈合。

Objective To investigate the effects mechanism of skin regeneration medical technique （Moist expose burn therapy/Moist expose burn ointment,MEBT/MEBO）on TNF-αand IL-6 in the granulation tissues in the chronic non-healing skin wounds of rats.Methods Fifty-four SD rats were randomly divided into the observation group,rb-BFGF group and model group.After making the successful models of skin ulcer,the rats in the above groups were respectively posted with two layers of MEBO gauze,bFGF impregnated gauze,and saline gauze.In the blank group,we established models with full-thickness skin defect,without any intervention.Five rats were randomly chosen and killed separately 3,7 and 14 days in every group after the modeling.The granulation tissues in the same part of the skin ulcer wound were ob-tained and the histological structures were detected by HE staining.TNF-αand IL-6 expression was examined by ELISA technique.Results A large amount of micro angiogenesis generated in rats of the observation group and rb-BFGF group 14 days after the treatment.Besides,the inflammation cells disappeared and the skin wounds recovered well.The content of TNF-αand IL-6 was gradually decreased in all groups,the decline in the observation group was the most obvious.During the modeling,the expression of TNF-αand IL-6 in the observation group and rb-BFGF group was always higher than that in the blank group and the difference was statistically significant （all P 〈0.01）.Seven days after modeling,the expression of TNF-αand IL-6 in the observation group was lower than that in the rb-BFGF group and the difference is statistically signifi-cant （P 〈0.01）.Conclusion MEBT/MEBO could control the release of TNF-αand IL-6,change the pathological state＆amp;nbsp;of inflammation so as to promote the wound healing.