HPLC法测定麦冬及环境中多效唑的残留量

Determination of Paclobutrazol Remained in MaiDong and Its Growth Environment by HPLC

目的：确立麦冬及其生长环境中多效唑残留的快速检测方法。方法：采用无水甲醇,超声30分钟提取麦冬样品及其生长环境中多效唑。采用Agilent-C18色谱柱,流动相为甲醇-乙腈-0.1%甲酸（50：15：35）,流速1.0 m L/min;检测波长220 nm,柱温30℃。结果：在0.000 2~0.015μg之间线性良好,相关系数为0.999 6,回收率在98.6%~105.6%之间,RSD值为2.9149%,精密度实验、稳定性考察和重现性实验的RSD分别为0.998 5%、1.368 9%、1.008 3%。结论：本方法可快速、简便、准确地测定麦冬植株样品及其生长环境中的多效唑残留量。

Objective：To establish rapid determination method of paclobutrazol remained in MaiDong[Ophiopogon japonicus（Linn.f.） Ker-Gawl.].Methods：Paclobutrazol remained in MaiDong was extracted by ultrasound for30 minutes in absolute methanol.Agilent-C_（18） chromatographic column was adopted,mobile phase：methanol- acetonitrile-0.1%methanoic acid（50：15：35）,flow rate：1.0mL/min;detection wavelength was 220 nm,column temperature：30℃.Results：It showed better linear relationship between 0.000 2 and 0.015 μg,correlation coefficient was0.999 6,recovery rate was between 98.6%and 105.6%,RSD=2.9149%,RSD of precision,stability and reproducibility tests were 0.998 5%,1.368 9%and 1.008 3%.Conclusion：The method could be used to determine residual quantity of paclobutrazol in MaiDong and its growth environment accurately,simply and rapidly.