正常皮肤与增生性瘢痕中Ⅰ型和Ⅲ型胶原的含量与比例

Contents and ratios of type Ⅰ and type Ⅲ collagens in normal skin and hypertrophic scar in people of different ages

目的测定各年龄组人正常皮肤及增生性瘢痕中Ⅰ、Ⅲ型胶原的含量及比值,分析其变化规律和原因。方法采集流产胎儿的皮肤标本及儿童青少年组、成年组、老年组的正常皮肤标本和增生性瘢痕标本,采用免疫组织化学染色法测定Ⅰ、Ⅲ型胶原比值,羟脯氨酸法测定胶原总量,计算Ⅰ、Ⅲ型胶原的含量及比值。结果不同年龄组患者的正常皮肤比较,Ⅰ、Ⅲ型胶原含量的差异有统计学意义(P=0.01),Ⅰ、Ⅲ型胶原的比值呈上升趋势(P=0.02)。各年龄组的增生性瘢痕相比较,Ⅰ、Ⅲ型胶原含量和比值之间差异有统计学意义(P=0.01),两两比较,任意两组之间的差异有统计学意义(P=0.03)。各年龄组之中瘢痕与正常皮肤比较,Ⅰ、Ⅲ型胶原含量和比值间差异有统计学意义(P=0.02)。结论 1随着年龄的增长,人皮肤中Ⅰ、Ⅲ型胶原比值的变化导致皮肤结构和愈合过程发生改变;2在增生性瘢痕中,Ⅰ、Ⅲ型胶原含量和比例发生显著变化,导致瘢痕组织结构的不同。

Objective To quantify the contents of type I and III collagens and their ratios in normal skin and hypertrophic scar in people of different ages,and to explore the rule and cause of changes. Methods Normal human skin specimens and hypertrophic scar specimens were obtained from spontaneously aborted fetus and people of different ages,including the children and adolescent group（≤18 years）,adult group（19-50 years）,elderly group（ 〉50 years）.The total collagen contents were determined by hydroxyproline method. The ratios of types I and III collagens were examined by immunohistochemistry,and their contents were calculated. Results The difference of the contents of types I and III collagens and their ratios in normal skin were statistically significant among different age groups（P = 0. 01）.Their ratios increased with the increase of age,and the differences were statistically significant among different age groups（P = 0. 02）. The difference of contents of types I and III collagens and their ratios in hypertrophic scars were statistically significant among all groups,also between any two groups（P = 0. 03）. In every age group,the difference of the contents of types I and III collagens and their ratios between normal skin and hypertrophic scar was statistically significant（P = 0. 02）. Conclusion With age increasing,the changes of the ratios of types I and III collagens in the normal skin changes,which results in changes of the tension and elasticity of skin and the healing process after injury.The contents of types I and III collagens and their ratios significantly changes in hypertrophic scars,resulting in different structure of scar from normal skin.