益肾健脾方对少弱精子症小鼠模型睾丸生精功能和血清性激素水平的影响

Influences of Yishen Jianpi recipe on spermatogenesis function and serum sex hormone level of the mice model of oligoasthenotspermia

目的观察益肾健脾方对雷公藤多苷诱导少弱精子症小鼠模型睾丸组织、精液质量和血清性激素水平的影响,探讨益肾健脾方治疗少弱精子症可能的作用机制。方法以雷公藤多苷诱导少弱精子症小鼠模型,随机平均分为正常组、模型组、左卡尼汀组、中药组(益肾健脾方低、高剂量组),每组10只,中药及左卡尼汀干预,睾丸组织病理切片、HE染色,观察小鼠睾丸组织病理学,应用伟力精子测试仪检测小鼠精液质量,应用酶联免疫夹心法检测小鼠血清性激素水平。结果左卡尼汀组、中药组的A级精子活力、总活力高于模型组(P<0.05);左卡尼汀组低于中药低剂量组(P<0.05);中药高剂量组与左卡尼汀组相比差异无统计学意义(P>0.05)。左卡尼汀组、中药组的精子总数高于模型组(P<0.05);左卡尼汀组、中药低、高剂量组之间相比差异无统计学意义(P>0.05)。模型组小鼠血清卵泡刺激素、黄体生成素水平高于正常组及各药物干预组(P<0.05);各药物干预组高于模型组(P<0.05);各药物干预组之间相比差异无统计学意义(P>0.05);模型组及各干预组血清睾酮低于正常组(P<0.05);模型组与各干预组之间相比差异无统计学意义(P>0.05)。结论益肾健脾方可能通过调节小鼠血清性激素水平从而改善精液质量达到治疗少弱精子症的目的。

Objective To observe the changes of the testis tissue, semen quality and serum sex hormone level in mice model of oligoasthenotspermia induced by tripterygium glycosides, and discuss the possible intervention mechanism of Yishen Jianpi recipe to oligoasthenotspermia. Methods Mice model of oligoasthenotspermia was induced by tfipterygium glycosides and the mice were randomly divided into normal group, model group, L-carnitine group, Chinese medicine group（ Yishen Jianpi recipe low-dose and high-dose group）each group inclucled 120 mice. Chinese medicine and L-caruitine were used for intervention, Pathological section HE staining and electron microscope were used to observe the pathological changes of tissue, Weili sperm tester were used to observe the semen quality, serum sex hormone levels were detected by enzyme linked immunosorbent assay. Results A level and total sperm motility in the L-carnitine group and Chinese medicine group were higher than those in the model group （ P〈0.05 ） ; the L-carnitine group were lower than the low-dose group （ P〈0. 05 ） ; compared with the L-carnitine group, the high-dose group was not significant difference （P〉0.05）. Sperm total in the L-carnitine group and Chinese medicine group were higher than that in the model group （ P〈0.05 ） ; among the L-carnitin group, low-dose and high-dose group, there were no statistical significance（P〉0.05）. Serum follicle stimulating hormone and lutein level in other group were higher than that in the normal group （ P〈0. 05 ） ; intervention groups were higher than model group（P〈0.05） ; there was no significance difference between model group and in- tervention groups （P〉0. 05 ）. There was no significance difference in serum T level between the model group and intervention groups （P〉0.05） ; the model group and intervention group were lower than that in the normal group （ P 〈 0. 05 ）. Conclusion Yishen Jianpi recipe can regulate the level of serum sex hormone in mice model of oligoasthenotspermia and affect the occurrence of sperm to achieve the goal of im- proving the quality of semen in the treatment of oligoasthenotspermia.