FBXO31对肺癌细胞增殖、克隆形成、迁移及侵袭能力的影响

Effect of FBXO31 on proliferation, clone formation, migration and invasion of lung cancer cells

目的探讨FBXO31基因对肺腺癌细胞系A549增殖、克隆形成、迁移及侵袭能力的影响。方法通过脂质体转染法将FBXO31表达质粒及FBXO31 si RNA序列分别转染肺癌A549细胞,用Western blot、MTT、平板克隆、划痕和Transwell实验分别检测A549细胞中FBXO31蛋白表达、细胞增殖、克隆形成、迁移和侵袭能力。结果 Western blot显示FBXO31表达质粒转染上调A549细胞中FBXO31蛋白表达,而转染si RNA下调FBXO31蛋白表达。FBXO31基因过表达增强A549细胞增殖、克隆形成、侵袭及迁移能力,而FBXO31基因沉默抑制细胞增殖、克隆形成、侵袭及迁移能力(P<0.01)。结论 FBXO31基因可促进肺癌细胞增殖、克隆形成、迁移及侵袭能力。

Objective To investigate the effect of FBXO31 on proliferation, clone formation, migration and invasion of lung cancer cells. Methods FBXO31 expressing plasmids and siRNAs were respectively transfected into A549 cells using lipofection. FBXO31 expression, proliferation, colony formation, migration, and invasion ofA549 cells were determined by Western blot, MTT, plate clone formation, wound scratch and Transwell assays, respectively. Results Western blot showed that FBXO31 plasmid transfection upregulated FBXO31 expression, while FBXO31 siRNAs downregulated FBXO31 expression. FBXO31 gene overexpression enhanced the proliferation, colony formation, migration and invasion ofA549 cells, but FBXO31 gene silence inhibited these capacities （P〈0.01）. Conlusion FBXO31 gene can promote the proliferation, colony formation, migration and invasion ofA549 cells.