尕海湖湿地泥炭细菌多样性分析

Peat bacterial diversity and community structure in Gahai Lake wetlandin Gan'nan

【目的】探讨甘南尕海自然保护区湿地泥炭细菌的多样性和群落结构,通过3个不同地点的随机取样,对尕海自然保护区尕海湖的泥炭资源进行细菌多样性分析。【方法】运用Illumina Mi Seq高通量测序平台,对尕海湖湿地泥炭的细菌群落进行宏观基因组测序分析,检测了泥炭3个样本的细菌群落的差异性。【结果】通过对Silva数据库的比对,总共获得了108 096条优化序列代表。尕海自然保护区湿地泥炭样本的优势细菌群主要为Anawerodineaceae-uncultured(厌氧绳菌目-不可培养)、Micromonospora(小单孢菌属)、Brevundimong(短波单孢菌属)和Nocardioides(诺卡氏菌属),3个样品细菌群落组成差异较大。【结论】首次阐明了尕海湖水陆过渡区泥炭地细菌群落的多样性,为下一步开发和利用泥炭的功能菌群、研究泥炭微生物群落生态网络结构如何响应环境变化提供了基础资料。

[Objective] In order to identify peat bacterial diversity and community structure in Gahai Lake wetland in Gan＇nan. [Methods] By Illumina Mi Seq high-throughput DNA sequencing platforms, we studied the bacterial diversity from three samples of peat colonies. [Results] We obtained 108 096 non-redundant sequences by comparing the silva databases. The preponderant bacterium from three Gahai Lake wetland peat samples were Anawerodineaceae-uncultured, Micromonospora, Brevundimong and Nocardioides. [Conclusion] Peat bacterial diversity and community structure in Gahai Lake wetland can contribute to the development and utilization of functional bacteria.