玉米内生细菌的动态分布及拮抗作用

Dynamic Distribution and Antagonism of Endophytic Bacteria from Maize

[目的]比较不同品种及生育期玉米内生细菌的多样性差异,对分离到的内生细菌的拮抗作用进行鉴定。[方法]以桂单0810、迪卡008、正大619玉米品种苗期和穗期的不同组织为研究对象,采用组织研磨匀浆方法分离培养内生细菌,结合形态生理特征和16S r DNA基因序列同源性比对分析对菌株进行了分类,并测定了所分离的菌株对4种玉米病原菌的拮抗作用。[结果]从桂单0810、迪卡008、正大619这3个玉米品种苗期和穗期的根、茎、叶中共分离到82株内生细菌,归于4个属,芽孢杆菌属为优势菌群,各菌群在不同生育期及组织的分布动态各有特点,其中有8株菌对4种玉米病原菌表现出不同程度的拮抗作用。[结论]玉米内生细菌的多样性丰富,可作为玉米病害生防菌的来源。

[Objective] The aim was to compare the endophytic bacteria diversity and antagonism function isolated from different maize varieties at different growth stages.[Method] With different tissues of Guidan0810,Dika008,Zhengda 619 at seedling stage and heading stage as research objects,endophytic bacteria were isolated and cultured by tissues homogenate.Strains were classified combined with morphological and physiologi-cal characteristics and sequence homology comparison of 16S rDNA gene,the antagonism ability of isolated strains to 4 maize pathogens were iden-tified.[Result] Totally 82 strains which belonged to 4 genus were isolated from root,stem and leaf at seedling stage and heading stage of maize variety Guidan 0810,Dekarl 008 and Zhengda 619,and Bacillus was the dominant flora.The bacterial population composition was diverse from the growth stage and tissues.8 strains were showing antagonism ability to 4 pathogens.[Conclusion] The maize endophytic bacteria diversity is abun-dant,and can be the source of biocontrol bacterium for maize disease .