超高压高效液相色谱法测定茶酒中茶氨酸含量

Determination of Theanine in Tea Liquor by Ultra High Pressure and High Performance Liquid Chromatography

[目的]研究茶酒中茶氨酸含量的分析方法。[方法]以邻苯二甲醛（ OPA）为衍生剂，采用超高压高效液相色谱测定茶酒中的茶氨酸。[结果]检测茶氨酸的线性范围为0．1～100．0μg／mL，线性方程可拟合为Y＝53．57X－41．664，相关系数r为0．99959，该方法的最低检出限达到0．05μg／g（以S／N＝3计）。样品中茶氨酸的含量为0．25 mg／kg，加标试验的回收率在99．6％～100．1％。[结论]该方法快速、简单、定量准确，可在茶氨酸的测定分析中推广应用。

[Objective] The analytical method for content of theanine in tea liquor was investigated.[Method] Using o-phthalaldehyde （OPA） as a derivating agent,a method was established for determination of theanine in tea liquor by super high-pressure high performance liquid chromatog-raphy.[Result] The results showed that the linear range of 0.1-100.0 μg/mL for theanine detection was obtained,and the corresponding linear equation was fitted as Y=53.57X-41.664 with correlation coefficient r of 0.999 59.Also,the minimum detection limit of 0.05 μg/g （S/N=3） was reached.The theanine content of the sample was determined as 0.25 mg/kg with a recovery rate of 99.6%-100.1%.[Conclusion] The method is rapid,simple and accurate,which is easy to be widely used in the determination and analysis of theanine in tea liquor .