LAT52启动子在甘蓝花药中的表达模式

Expression Profile of LAT52 Promoter in Anther of Brassica oleracea L.

[目的]验证花药特异性启动子LAT52能否在甘蓝花药中发挥功能。[方法]从番茄中扩增得到LAT52核心序列608 bp,进行序列分析,连接LAT52启动子到双元表达载体p BI121,转化农杆菌GV3101,用农杆菌浸染法侵染甘蓝幼苗下胚轴,获得2株阳性植株,对转基因甘蓝植株的花蕾、花药及花粉进行GUS染色。[结果]LAT52核心序列608 bp具多个与花粉特异性表达相关的元件,浸染的LAT52启动子仅在花药及花粉中表达出蓝色,其他部位未染上蓝色。[结论]LAT52启动子仅在甘蓝花药及花粉中特异性表达。

[Objective] To verify whether specific promoter LAT52 could play a function in anther of cabbage.[Method] LAT52 core se-quence 608 bp was obtained from tomato by amplification technology, the sequence was analyzed, the LAT52 promoter was connected to the bi-nary expression vector pBI121, Agrobacterium GV3101 was transformed, Agrobacterium infection method was used to infect hypocotyl of cab-bage seedling, and 2 positive plants were obtained, transgenic cabbage plants flower bud, anther and pollen was stained by GUS.[Result] The LAT52 core sequence 608 bp had multiple pollen specific expression elements .The infected LAT52 promoter only expressed blue in the anther and pollen, and the other parts were not stained blue .[Conclusion] The LAT52 promoter can be expressed in anther and pollen of cabbage .