番红花球茎组培中的消毒和褐化抑制方法

Methods for Disinfection and Browning Inhibition of Crocus sativus L. Corm in Tissue Culture

[目的]优化番红花球茎消毒和褐化抑制的方法。[方法]以番红花球茎的染菌率、褐化率和愈伤组织诱导率为指标,分析了外植体大小、氯化汞浓度和消毒时间对番红花球茎染菌和诱导的影响,同时比较了抗坏血酸和活性炭对番红花球茎褐化的抑制效果。[结果]当外植体大小5 mm^3,氯化汞溶液浓度0.2%、消毒时间10 min时,染菌率和诱导率较为适宜;向培养基中加入0.5 g/L活性炭可以降低球茎的褐化。[结论]该研究建立了番红花球茎消毒和褐化抑制方法,为野外番红花球茎的初代组培提供了理论依据。

[Objective] To optimize the methods for disinfection and browning inhibition of Crocus sativus L.corm.[ Method] With contami-nation rate, browning rate and callus induction rate of C.sativus corm as the indexes, we analyzed the effects of explant size, HgCl2 concentra-tion and disinfection time on the contamination and induction of corm.Meanwhile, the inhibition effects of ascorbic acid and active carbon on the browning of the corm were compared, respectively.[ Result] The contamination rate and the callus induction rate were suitable under the conditions of 5 mm3 explant size, 0.2% HgCl2 concentration and 10 min disinfection time.The browning rate of the corm was lowered as the active carbon （0.5 g/L） was added into the culture medium.[Conclusion] The methods for disinfection and browning inhibition provide cer-tain practical evidence for the original tissue culture of wild C.sativus corm.