寻常性银屑病的皮肤镜特征及促炎因子、TGF-β信号通路、凋亡分子的表达量

Features under dermoscopy as well as the expression of pro-inflammatory factors,TGF-β signaling pathway and apoptosis molecules of psoriasis vulgaris

目的：研究寻常性银屑病的皮肤镜特征及促炎因子、TGF-β信号通路、凋亡分子的表达量。方法：收集2012年5月-2015年12月就诊的134例在本院确诊为寻常性银屑病的皮损组织作为病理组,同期骨科手术时修剪的四肢新鲜正常皮肤作为对照组,通过皮肤镜观察病理组皮损组织的特征,测定病理组和对照组皮肤组织中促炎作用、TGF-β信号分子、凋亡相关分子的表达量。结果：寻常性银屑病患者皮肤镜下的典型特征为淡红色背景、点状血管形态、规则血管排列模式;病理组皮肤组织中IFN-γ、IL-12、IL-17、IL-18、c-myc、Bcl-2、Survivin的含量显著高于对照组（P〈0.05）,IL-4、IL-10、TGF-β1、TGF-β1R-I、TGF-β1R-II、Smad2、Smad3、PTEN、CEACAM1的含量显著低于对照组（P〈0.05）。结论：寻常性银屑病皮肤镜下特征为规则点状新生血管形成,皮损组织中Th1/Th2、Th17/Treg紊乱以及凋亡信号异常与银屑病的发生有关。

Objective：To study the features under dermoscopy as well as the expression of pro-inflammatory factors,TGF-βsignaling pathway and apoptosis molecules of psoriasis vulgaris.Methods：A total of 134 cases of skin lesion tissues diagnosed with psoriasis vulgaris in our hospital were collected as the pathological group,fresh normal limb skin tissues trimmed in the orthopaedic surgery during the same period were selected as control group,dermoscopy was used to observe the features of pathological group,and the expression levels of pro-inflammatory factors,TGF-βsignal molecules and apoptosis-related molecules in pathological group and control group were determined.Results：The typical features of patients with psoriasis vulgaris under dermoscopy were pink background,dotted vascular morphology and regular vascular arrangement pattern;IFN-γ,IL-12,IL-17,IL-18,c-myc,Bcl-2and Survivin content in pathological group were significantly higher than those in control group while IL-4,IL-10,TGF-β1,TGF-β1R-I,TGF-β1R-II,Smad2,Smad3,PTEN and CEACAM1 content were significantly lower than those in control group.Conclusion：The feature of psoriasis vulgaris under dermoscopy is regular dotted angiogenesis,and the Th1/Th2 and Th17/Treg disorder as well as abnormal apoptotic signals in skin lesion tissue are associated with the occurrence of psoriasis.