同源异体骨髓间充质干细胞移植治疗犬急性肝功能损伤

Treatment of Acute Liver Injury in Dogs by Allogeneic Transplantation of Bone Marrow Mesenchymal Stem Cells

探讨同源异体骨髓间充质干细胞(BMMSCs)移植对犬急性肝功能损伤的治疗作用,为犬干细胞治疗相关疾病提供理论基础和技术支持。对患急性肝损伤的10只病犬经超声引导腹腔内肝门附近移植BMMSCs,移植后检测血常规、肝功能和肝脏B超,观察同期的症状体征和不良反应情况。结果显示,干细胞移植7天后可使患犬各项肝功能指标明显改善,10d后B超结果显示肝组织回声均匀,无明显异常,14d后患犬的精神、体力、食欲明显好转,干细胞移植21d后血常规各项指标恢复正常,患犬基本康复,移植干细胞的患犬均未发现有不良反应。说明相对于常规治疗,犬BMMSCs同源异体移植治疗急性肝损伤可明显缩短治疗时间,显著提高患犬生活质量。

This study was designed to investigate the allogeneic transplantation bone marrow mesenchymal stem cells（BMMSCs）for treatment on canine acute hepatic injury,which provided a theoretical basis and technical support for the clinical application of canine stem cell transplantation.The canine BMMSCs were transplanted to treat acute liver injury of 10 dogs by ultrasound-guided near hepatic portal in abdomen,the routine blood,the liver function and liver B ultrasound were detected after transplantation,and the simultaneous symptoms and adverse reactions were observed.The results showed that routine blood test indexes returned to normal after 21 days transplantation,various liver function indexes were ameliorated significantly after 7days transplantation.B ultrasound results showed that the liver tissue echoed uniformly,no obvious abnormalities after 10 days,and the spirit,strength,appetite of dogs was improved evidently after14 days,all dogs were recovered after 21 days and no adverse reaction were observed.Compared to the conventional treatment,the results indicated that canine BMMSCs allogeneic transplantation for treatment canine acute liver injury in dogs was safe and effective and no side effects,and could enhanced life quality of dogs significantly.