葛根芩连汤治疗对2型糖尿病湿热证肠道菌群影响

Effect of Intestinal Flora of Type 2 Diabetes Patients with Dampness-heat Syndrome Treated with Gegen Qinlian Decoction

目的:观察葛根芩连汤治疗2型糖尿病湿热证的疗效及对患者肠道菌群的影响。方法:将42例2型糖尿病湿热证患者随机分两组,对照组使用单纯西药治疗,中药组在西药治疗的基础上联用葛根芩连汤。采用Illumina Miseq高通量测序技术对患者粪便样本中所有细菌的16S rRNA-V3区进行DNA测序,通过实时定量PCR对肠道菌群进行定量分析。对比观察两组患者治疗前后疗效与肠道菌群的关系。结果:中药组总有效率高于对照组,差异具有统计学意义(p=0.02)。湿热证减分率和Bacteroides含量成负相关;湿热证减分率和Fusobacterium含量呈正相关。结论:葛根芩连汤联合西药可有效治疗湿热证2型糖尿病,Bacteroides和Fusobacterium含量变化可作为疗效观察的标志物。

Objective：To observe the effect of Gegen Qinlian Decoction in treating type 2 diabetes pa-tients with dampness-heat syndrome and the effect of intestinal flora. Methods：Forty-two type 2 diabetes pa-tients with damp-heat syndrome were randomly divided into 2 groups：the control group was treated with West-ern medicine alone, and the experimental group was treated with integrated Traditional and Western medicine. The fecal samples were collected to extract bacterial genome DNA, and the composition was investigated by Il-lumina Miseq high-throughput sequencing of the V3 region of the 16S rRNA gene. Species in feces were also measured by real-time quantitative PCR. The relationship between effectiveness and gut microbiota was ob-served after treatment. Results： The total effectiveness of experimental group was higher than that of control group. Bacteroides correlated negatively with the reduction rate of dampness-heat syndrome, while Fusobacte-rium correlated positively with the reduction rate of dampness-heat syndrome. Conclusion：The combination of Gegen Qinlian Decoction with Western medicine has the effective treatment among type 2 diabetes patients with dampness-heat syndrome, and Bacteroides and Fusobacterium could be the markers of effectiveness.