陕西秦岭地区三叶木通遗传多样性的AFLP分析

AFLP analysis on genetic diversity of Akebia trifoliate in Qinling Mountains of Shaanxi

目的利用扩增片段长度多态性(AFLP)分子标记技术对陕西秦岭地区6个天然居群的三叶木通种质进行遗传多样性分析。方法利用10对引物组合对111份三叶木通种质进行AFLP扩增,并评估遗传多样性。结果 AFLP扩增结果共产生221条清晰的条带,其中多态性条带132条,占59.7%。6个三叶木通居群间遗传分化系数为0.505,基因多态性指数为0.185。在Nei’s遗传距离0.13处,6个天然居群被聚为4个类群。结论陕西秦岭地区三叶木通天然居群遗传多样性水平较低,居群内与居群间的遗传变异程度基本相同。

Objective The genetic diversity ofAkebia trifoliate germplasm from six different populations in Qinling Mountains was studied by AFLP molecular marker. Methods The amplification bands were obtained by using l0 primer combinations and genetic diversity were estimated for 111 A. trifoliate germplasm. Results A total of 221 clear bands were obtained, of which 132 were polymorphic bands and the percentage of polymorphic was 59.7%. Among the six populations, the genetic differentiation coefficient was 0.505, gene polymorphism index was 0.185. The six natural populations can be divided into four groups at Nei＇s genetic distance of 0.13. Conclusion The populations ofA. trifoliate in Qinling Mountains of Shaanxi exist a low level of genetic diversity, almost the same genetic variation occurs within and among populations.