洗发、护发化妆品微生物检查方法学研究

Microbial Limit and Microbe-Controlling Method for Shampoos and Hair Conditioners

为提高化妆品潜在微生物的阳性检出率,建立洗发、护发类化妆品微生物限度和控制菌检查方法。采用常规法、培养基稀释法、薄膜过滤法对4种洗发、护发类化妆品进行微生物限度与控制菌方法学研究。结果显示,飘柔长效柔顺滋养洗发露、海飞丝去屑洗发露、飘柔人参滋养润发精华素、力士密集滋养修复-发膜级精华素菌落总数检测方法分别为0.2 m L/皿法、800 m L/膜法、0.5 m L/皿法和300 m L/膜法;霉菌及酵母菌检测方法分别为300 m L/膜法、300 m L/膜法、1 m L/皿法和1 m L/皿法;除海飞丝去屑洗发露采用培养基稀释法,其他均采用常规法进行控制菌检查。建议在化妆品微生物检验前应进行微生物方法研究,从而提高化妆品"潜在"病原菌的检出率。

In order to improve the positive detection rate of potential microbe in cosmetics,a microbial limit and microbe-controlling method for shampoos and hair conditioners was established. Routine method,medium diluting method,membrane filtration method was adopted for the study of microbial limit and microbe-controlling method. The results showed that bacterial count of Rejoice Long-lasting Soft Nourishing Shampoo,Head and Shoulders Antidandruff Shampoo,Rejoice Ginseng Nourishing Essence,Lux Intensive Nourishing Repairment Essence should be tested by the method of 0. 2 m L / dish method（ dm）,800 m L / membrane method（ mm）,0. 5 m L / dm and 300 m L /mm,respectively. Yeast and mold count were 300 m L / mm,300 m L / mm,1 m L / dm and 1 m L / dm; by routine method and routine method. Microbe-controlling except for Head and Shoulders Anti-dandruff Shampoo was tested by medium diluting method,the other were all using routine method to carry out the detection of micro-controlling.Therefore,it is suggested that before verifying microbial method,study on microbial method should be carried out,thus improving detection rates of potential microbe in cosmetics.