大黄牡丹汤组方对急性胰腺炎模型大鼠胰腺微循环的影响

The Effect of Dahuang Mudan Decoction to Pancreatic Gland Microcirculation of Acute Pancreatitis Model Rat

目的研究大黄牡丹汤组方(RPDP)对急性胰腺炎(AP)模型大鼠胰腺早期胰腺微循环的改变,探讨微循环改变在AP发生发展中的作用,观察RPDP对胰腺微循环的影响。方法 60只SD大鼠随机分为药物作用4 h假手术(SO)组、模型组和RPDP组;药物作用8 h SO组、模型组和RPDP组,每组各10只。模型组和RPDP组在距离胆胰管开口0.3 mm处的胆管,经十二指肠壁浆肌层针刺,用3.5%牛磺胆酸钠0.1 m L/100 g缓慢注射,建立SD大鼠AP模型,治疗组用RPDP 40 g/kg灌胃1次。分别与给药后4 h和8 h,麻醉相应组大鼠抽取血液及腹水,检测各组血清和腹水淀粉酶含量。用荧光显微流速测试方法,观察胰腺微血管在造模后4 h和8 h的变化。结果模型组大鼠胰腺微循环血流速度存在差异,RPDP治疗组血流速度则比较一致。与SO组比较,模型组大鼠血清淀粉酶及腹水淀粉酶均显著升高;与模型组比较,RPDP组大鼠血清淀粉酶及腹水淀粉酶均显著降低(P<0.01)。结论 AP发病与胰腺微循环障碍有关,AP向重症急性胰腺炎(SAP)发展也与其有关,AP时胰腺水肿与坏死同时存在。RPDP有改善AP模型大鼠胰腺微循环的作用。

Objective To study the effect of Dahuang Mudan Decoction to pancreatic gland microcirculation of acute pancreatitis model rat,investigate the function of minicirculation alteration in acute pancreatitis. Method Divided 60 SD rats randomly into 6 groups as drug action 4 hours SO group,model group,RPDP group,and drug action 8 hours SO group,model group,RPDP group,there were 10 rats in each group. Rats in the model group and RPDP group,3. 5 % Sodium Cholate was retrogradedly injected into the pancreatic duct to produce the AP model. RPDP 40 g/kg intragastric administration 1 times in the RPDP group. 4 h and 8 h after intragastric administration,to get the blood and ascites, detect the content of amylase in the blood and ascites. Use fluorescence microscopy flow rate test method,observe the change of pancreatic gland microcirculation 4 h and 8 h after administration. Result There are blood flow rate difference of pancreatic gland microcirculation in the model group,and the blood flow rate in RPDP group are coincidence. Compared with the SO group,the serum-amylase and ascites-amylase were all significantly elevated in the model group. Compared with the model group,the serumamylase and ascites-amylase were all significantly depress（ P 〈 0. 01）. Conclusion AP was concerned with pancreatic gland microcirculation disturbance, pancreatic gland edema and pancreatic gland necrosis were simultaneous in AP. RPDP could improve pancreatic gland microcirculation disturbance in AP modle rats.