实时荧光PCR法和病毒分离培养法在手足口病病毒检测中的应用分析

The application of real-time PCR and virus isolation for detection of hand foot and mouth disease virus

目的筛选快速准确的检测方法,为手足口病监测与防控工作提供及时可靠的病原学依据。方法采用病毒分离培养法和实时荧光PCR检测法对2014年牡丹江市手足口病监测哨点医院采集的手足口样病例标本进行检测,比较检测结果。结果 2014年采集的206份标本中,实时荧光PCR法检测肠道病毒EV 71型42例、Cox A16型9例、肠道病毒通用型37例,阴性118例。病毒分离培养法检测肠道病毒EV 71型4例、Cox A16型4例、肠道病毒通用型6例。实时荧光PCR法、病毒分离培养法检测阳性率分别为42.72%和6.80%;与实时荧光PCR法相比,病毒分离培养法灵敏度、特异度分别为15.91%、100.00%。结论实时荧光PCR法比病毒分离培养法更灵敏快捷、特异性强、敏感度高等,适用于手足口病病原体的检测。

Objective To compare the efficacy of the Methods of the virus isolation and culture and real - time PCR for detec- tion of hand foot and mouth disease （HFMD） virus in order to screen accurate and rapid influenza virus detection meth- ods. Methods The HFMD virus from HFMD - Like patients were detected by using Methods of virus isolation and culture, and real - time PCR then the Results were compared. Results Totally, 206 specimens were collected during 2014, and 42 were i- dentified as EV 71, 9 cases of Cox A16, 37 general type of enterovirus and t18 negative with real - time PCR. The virus isola- tion and culture detected 4 EV 71 - positive samples, 4 Cox A16 - positive samples and 6 other enterovirus. ＇File positive ratios for real - time PCR and the virus isolation and culture were 42. 72% and 6. 80% respectively. Compared with real - time PCR, the sensitivity and specificity for the virus isolation and culture were 15. 91% and 100.00%, respectively. Conclusion Real - time PCR was more faster, high sensitivity and specificity than the virus isolation and culture, so real -time PCR is more suit- able for detection of HFMD in specimens.