灰树花多糖螯合铁的制备工艺研究

Study on preparation process of Grifola frondosa polysaccharide-iron complex

目的对灰树花多糖和三价铁Fe(Ⅲ)的螯合工艺进行初步研究,确定最佳螯合条件。方法灰树花粗多糖用Sevag法除蛋白后,在碱性条件下与三氯化铁进行反应制得灰树花多糖与Fe(Ⅲ)的螯合物,用邻菲啰啉法测定铁含量,考察灰树花多糖与柠檬酸三钠的比例、螯合温度、螯合时间和溶液pH 4个因素对产品中铁含量的影响,然后通过正交实验确定最佳螯合条件。结果灰树花多糖与柠檬酸三钠的比例对螫合效果的影响最大,其次是螯合时间和螯合温度,溶液pH的影响最小,螯合的最佳工艺条件为灰树花多糖与柠檬酸三钠的比例为2:1,螯合温度为50℃,螯合时间为0.5 h,溶液pH为8。经验证,此条件下制得的螯合物中铁含量最高,为3.08%。结论本研究的制备工艺条件可以实现灰树花多糖与Fe(Ⅲ)的有效螯合,制得的灰树花多糖螯合铁有望成为一种新型的补铁剂。

Objective To study the chelating process of the polysaccharide from Grifola frondosa and Fe（III）,and determine the optimum chelate condition.Methods The action of polysaccharide from Grifola frondosa and iron trichloride was conducted under alkaline conditions after deproteininzation by Sevag method,and the iron content in product was detected by phenanthroline method.The effects of mass ratio of polysaccharide from Grifola frondosa to trisodium citrate,chelating time,chelating temperature and pH value of solution on iron content in product were investigated by the single factor experiments,and the optimum chelating condition was determined by the orthogonal experiment.Results The mass ratio of polysaccharide from Grifola frondosa to trisodium citrate on iron content in chelate was the most influential factor,followed by chelating time,chelating temperature and pH value of solution.The optimum chelating condition were as follows：mass ratio of polysaccharide from Grifola frondosa to trisodium citrate was 2：1,chelating temperature was 0.5 h,chelating temperature was 50 ℃,and pH value of solution was 8.The content of iron was the highest（3.08%） under the above conditions.Conclusion The effective chelating of the polysaccharide from Grifola frondosa and Fe（Ⅲ） can be achieved by this process,and the Grifola frondosa polysaccharide-iron complex is expected to be a new type of iron supplement.